Matrix-assisted laser desorption ionization imaging mass spectrometry: in situ molecular mapping

Matrix-assisted laser desorption ionization imaging mass spectrometry (IMS) is a relatively new imaging modality that allows mapping of a wide range of biomolecules within a thin tissue section. The technology uses a laser beam to directly desorb and ionize molecules from discrete locations on the t...

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Vydané v:Biochemistry (Easton) Ročník 52; číslo 22; s. 3818
Hlavní autori: Angel, Peggi M, Caprioli, Richard M
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 04.06.2013
Predmet:
Animals
Breast Neoplasms - chemistry
Embryo, Mammalian - chemistry
Fixatives
Lung - chemistry
Mice
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Tissue Array Analysis - methods
ISSN:1520-4995, 1520-4995
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Popis
Shrnutí:Matrix-assisted laser desorption ionization imaging mass spectrometry (IMS) is a relatively new imaging modality that allows mapping of a wide range of biomolecules within a thin tissue section. The technology uses a laser beam to directly desorb and ionize molecules from discrete locations on the tissue that are subsequently recorded in a mass spectrometer. IMS is distinguished by the ability to directly measure molecules in situ ranging from small metabolites to proteins, reporting hundreds to thousands of expression patterns from a single imaging experiment. This article reviews recent advances in IMS technology, applications, and experimental strategies that allow it to significantly aid in the discovery and understanding of molecular processes in biological and clinical samples.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
ObjectType-Review-3
content type line 23
ISSN:1520-4995
1520-4995
DOI:10.1021/bi301519p