Quantitative, high-resolution proteomics for data-driven systems biology

Systems biology requires comprehensive data at all molecular levels. Mass spectrometry (MS)-based proteomics has emerged as a powerful and universal method for the global measurement of proteins. In the most widespread format, it uses liquid chromatography (LC) coupled to high-resolution tandem mass...

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Veröffentlicht in:Annual review of biochemistry Jg. 80; S. 273
Hauptverfasser: Cox, Jürgen, Mann, Matthias
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 01.01.2011
Schlagworte:
Chromatography, Liquid - methods
Humans
Peptides - analysis
Protein Processing, Post-Translational
Proteins - analysis
Proteome - analysis
Proteomics - methods
Systems Biology
Tandem Mass Spectrometry - methods
ISSN:1545-4509, 1545-4509
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Zusammenfassung:Systems biology requires comprehensive data at all molecular levels. Mass spectrometry (MS)-based proteomics has emerged as a powerful and universal method for the global measurement of proteins. In the most widespread format, it uses liquid chromatography (LC) coupled to high-resolution tandem mass spectrometry (MS/MS) to identify and quantify peptides at a large scale. This peptide intensity information is the basic quantitative proteomic data type. It is used to quantify proteins between different proteome states, including the temporal variation of the proteome, to determine the complete primary structure of proteins including posttranslational modifications, to localize proteins to organelles, and to determine protein interactions. Here, we describe the principles of analysis and the areas of biology where proteomics can make unique contributions. The large-scale nature of proteomics data and its high accuracy pose special opportunities as well as challenges in systems biology that have been largely untapped so far.
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ISSN:1545-4509
1545-4509
DOI:10.1146/annurev-biochem-061308-093216