Dye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications

Near-infrared (NIR) dye-sensitized upconversion nanoparticles (UCNPs) can broaden the absorption range and boost upconversion efficiency of UCNPs. Here, we achieved significantly enhanced upconversion luminescence in dye-sensitized core/active shell UCNPs via the doping of ytterbium ions (Yb3+) in t...

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Veröffentlicht in:ACS nano Jg. 10; H. 1; S. 1060 - 1066
Hauptverfasser: Wu, Xiang, Zhang, Yuanwei, Takle, Kendra, Bilsel, Osman, Li, Zhanjun, Lee, Hyungseok, Zhang, Zijiao, Li, Dongsheng, Fan, Wei, Duan, Chunying, Chan, Emory M, Lois, Carlos, Xiang, Yang, Han, Gang
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States American Chemical Society 26.01.2016
American Chemical Society (ACS)
Schlagworte:
Animals
Energy Transfer
Female
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Hippocampus - physiology
Hippocampus - ultrastructure
Indoles - chemical synthesis
Indoles - chemistry
Luminescent Measurements
Mice
Mice, Inbred BALB C
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Neurons - physiology
Neurons - ultrastructure
Optical Imaging - instrumentation
Optical Imaging - methods
Optogenetics - instrumentation
Optogenetics - methods
Patch-Clamp Techniques
Polymethyl Methacrylate - chemistry
Primary Cell Culture
Rats, Sprague-Dawley
Spectroscopy, Near-Infrared
Ytterbium - chemistry
Yttrium - chemistry
dye-sensitizing
upconversion nanoparticles
near-infrared
core/active shell structure
bioimaging
optogenetics
ISSN:1936-0851, 1936-086X
Online-Zugang:Volltext
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Beschreibung
Zusammenfassung:Near-infrared (NIR) dye-sensitized upconversion nanoparticles (UCNPs) can broaden the absorption range and boost upconversion efficiency of UCNPs. Here, we achieved significantly enhanced upconversion luminescence in dye-sensitized core/active shell UCNPs via the doping of ytterbium ions (Yb3+) in the UCNP shell, which bridged the energy transfer from the dye to the UCNP core. As a result, we synergized the two most practical upconversion booster effectors (dye-sensitizing and core/shell enhancement) to amplify upconversion efficiency. We demonstrated two biomedical applications using these UCNPs. By using dye-sensitized core/active shell UCNP embedded poly­(methyl methacrylate) polymer implantable systems, we successfully shifted the optogenetic neuron excitation window to a biocompatible and deep tissue penetrable 800 nm wavelength. Furthermore, UCNPs were water-solubilized with Pluronic F127 with high upconversion efficiency and can be imaged in a mouse model.
Bibliographie:ObjectType-Article-1
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content type line 23
AC05-76RL01830
PNNL-SA-115307
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Author Contributions
X. Wu, Y. Zhang, and K. Takle contributed equally to this work.
ISSN:1936-0851
1936-086X
DOI:10.1021/acsnano.5b06383