Efficient Biosynthesis of (2 S )-Naringenin from p -Coumaric Acid in Saccharomyces cerevisiae
(2 )-Naringenin, a (2 )-flavanone, is widely used in the food, chemical, and pharmaceutical industries because of its diverse physiological activities. The production of (2 )-naringenin in microorganisms provides an ideal source that reduces the cost of the flavonoid. To achieve efficient production...
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| Published in: | Journal of agricultural and food chemistry Vol. 68; no. 4; p. 1015 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
United States
29.01.2020
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| Subjects: | |
| ISSN: | 1520-5118, 1520-5118 |
| Online Access: | Get more information |
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| Summary: | (2
)-Naringenin, a (2
)-flavanone, is widely used in the food, chemical, and pharmaceutical industries because of its diverse physiological activities. The production of (2
)-naringenin in microorganisms provides an ideal source that reduces the cost of the flavonoid. To achieve efficient production of (2
)-naringenin in
(
), we constructed a biosynthetic pathway from
-coumaric acid, a cost-effective and more efficient precursor. The (2
)-naringenin synthesis pathway genes were integrated into the yeast genome to obtain a (2
)-naringenin production strain. After gene dosage experiments, the genes negatively regulating the shikimate pathway and inefficient chalcone synthase activity were verified as factors limiting (2
)-naringenin biosynthesis. With fed-batch process optimization of the engineered strain, the titer of (2
)-naringenin reached 648.63 mg/L from 2.5 g/L
-coumaric acid. Our results indicate that the constitutive production of (2
)-naringenin from
-coumaric acid in
is highly promising. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1520-5118 1520-5118 |
| DOI: | 10.1021/acs.jafc.9b05218 |