Self-Replicating Catalyzed Hairpin Assembly for Rapid Signal Amplification

A rapid signal amplification system based on the self-replicating catalyzed hairpin assembly is reported in which two hairpins, H1 and H2, were well-designed in which two split target/trigger DNA and two split G-quadruplex sequences were respectively integrated into H1 and H2. Target/trigger DNA can...

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Veröffentlicht in:	Analytical chemistry (Washington) Jg. 89; H. 22; S. 11971
Hauptverfasser:	Dai, Jianyuan, He, Hongfei, Duan, Zhijuan, Guo, Yong, Xiao, Dan
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 21.11.2017
ISSN:	1520-6882, 1520-6882
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Zusammenfassung:	A rapid signal amplification system based on the self-replicating catalyzed hairpin assembly is reported in which two hairpins, H1 and H2, were well-designed in which two split target/trigger DNA and two split G-quadruplex sequences were respectively integrated into H1 and H2. Target/trigger DNA can be cyclically used in this system to form the duplex DNA assemblies (H1-H2), which will bring the two G-quadruplex fragments into close-enough proximity to induce the formation of intact G-quadruplex as a colorimetric signal readout. Similarly, the two split target/trigger DNA sequences will reunite into a DNA sequence that is identical to the target/trigger DNA; then, the obtained replica can also be cyclically used as a new activator unit to trigger the CHA reaction, leading to the rapidly and significantly enhanced formation of target/trigger DNA replicas with the concomitant generation of a higher signal. This self-replication-based autocatalytic signal amplified approach has been successfully used to develop a rapid and visual assay for DNA and small molecule detection.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1520-6882 1520-6882
DOI:	10.1021/acs.analchem.7b01946