High-Resolution Shortwave Infrared Imaging of Vascular Disorders Using Gold Nanoclusters

We synthesized a generation of water-soluble, atomically precise gold nanoclusters (Au NCs) with anisotropic surface containing a short dithiol pegylated chain (AuMHA/TDT). The AuMHA/TDT exhibit a high brightness (QY ∼ 6%) in the shortwave infrared (SWIR) spectrum with a detection above 1250 nm. Fur...

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Published in:ACS nano Vol. 14; no. 4; pp. 4973 - 4981
Main Authors: Yu, Zhixi, Musnier, Benjamin, Wegner, K. David, Henry, Maxime, Chovelon, Benoit, Desroches-Castan, Agnès, Fertin, Arnold, Resch-Genger, Ute, Bailly, Sabine, Coll, Jean-Luc, Usson, Yves, Josserand, Véronique, Le Guével, Xavier
Format: Journal Article
Language:English
Published: United States American Chemical Society 28.04.2020
Subjects:
Animals
Bioengineering
Diagnostic Imaging
Gold
Imaging
Life Sciences
Light
Mice
Radio Waves
Water
shortwave infrared fluorescence
vascular disorder
Monte Carlo restoration imaging processing
bone morphogenetic protein 9 (Bmp9)
gold nanoclusters
ISSN:1936-0851, 1936-086X, 1936-086X
Online Access:Get full text
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Summary:We synthesized a generation of water-soluble, atomically precise gold nanoclusters (Au NCs) with anisotropic surface containing a short dithiol pegylated chain (AuMHA/TDT). The AuMHA/TDT exhibit a high brightness (QY ∼ 6%) in the shortwave infrared (SWIR) spectrum with a detection above 1250 nm. Furthermore, they show an extended half-life in blood (t 1/2ß = 19.54 ± 0.05 h) and a very weak accumulation in organs. We also developed a non-invasive, whole-body vascular imaging system in the SWIR window with high-resolution, benefiting from a series of Monte Carlo image processing. The imaging process enabled to improve contrast by 1 order of magnitude and enhance the spatial resolution by 59%. After systemic administration of these nanoprobes in mice, we can quantify vessel complexity in depth (>4 mm), allowing to detect very subtle vascular disorders non-invasively in bone morphogenetic protein 9 (Bmp9)-deficient mice. The combination of these anisotropic surface charged Au NCs plus an improved SWIR imaging device allows a precise mapping at high-resolution and an in depth understanding of the organization of the vascular network in live animals.
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ISSN:1936-0851
1936-086X
1936-086X
DOI:10.1021/acsnano.0c01174