Carbohydrate-based label-free detection of Escherichia coli ORN 178 using electrochemical impedance spectroscopy

A label-free biosensor for Escherichia coli (E. coli) ORN 178 based on faradaic electrochemical impedance spectroscopy (EIS) was developed. α-Mannoside or β-galactoside was immobilized on a gold disk electrode using a self-assembled monolayer (SAM) via a spacer terminated in a thiol functionality. I...

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Bibliographic Details
Published in:Analytical chemistry (Washington) Vol. 84; no. 1; p. 241
Main Authors: Guo, Xuefei, Kulkarni, Ashish, Doepke, Amos, Halsall, H Brian, Iyer, Suri, Heineman, William R
Format: Journal Article
Language:English
Published: United States 03.01.2012
Subjects:
Carbohydrates - chemistry
Dielectric Spectroscopy - methods
Escherichia coli - chemistry
Microscopy, Electron, Scanning
ISSN:1520-6882, 1520-6882
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Summary:A label-free biosensor for Escherichia coli (E. coli) ORN 178 based on faradaic electrochemical impedance spectroscopy (EIS) was developed. α-Mannoside or β-galactoside was immobilized on a gold disk electrode using a self-assembled monolayer (SAM) via a spacer terminated in a thiol functionality. Impedance measurements (Nyquist plot) showed shifts due to the binding of E. coli ORN 178, which is specific for α-mannoside. No significant change in impedance was observed for E. coli ORN 208, which does not bind to α-mannoside. With increasing concentrations of E. coli ORN 178, electron-transfer resistance (R(et)) increases before the sensor is saturated. After the Nyquist plot of E. coli/mixed SAM/gold electrode was modeled, a linear relationship between normalized R(et) and the logarithmic value of E. coli concentrations was found in a range of bacterial concentration from 10(2) to 10(3) CFU/mL. The combination of robust carbohydrate ligands with EIS provides a label-free, sensitive, specific, user-friendly, robust, and portable biosensing system that could potentially be used in a point-of-care or continuous environmental monitoring setting.
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ISSN:1520-6882
1520-6882
DOI:10.1021/ac202419u