Acid-polyacrylamide gel electrophoresis of lysozyme-estrone glucuronide conjugates

Acid-polyacrylamide gel electrophoresis (acid-PAGE) was used for analysis of lysozyme-estrone glucuronide conjugates. The resolution of the system allowed the identification of individual conjugate families which differed only in the position of acylation or in the number of estrone glucuronide unit...

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Bibliographic Details
Published in:Bioconjugate chemistry Vol. 9; no. 6; p. 838
Main Authors: Smales, C M, Elgar, D F, Moore, C H, Blackwell, L F
Format: Journal Article
Language:English
Published: United States 01.11.1998
Subjects:
Cytochrome c Group - metabolism
Electrophoresis, Polyacrylamide Gel
Estrogens, Conjugated (USP) - chemistry
Estrone - chemistry
Glucuronates - chemistry
Muramidase - chemistry
Protein Denaturation
ISSN:1043-1802
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Summary:Acid-polyacrylamide gel electrophoresis (acid-PAGE) was used for analysis of lysozyme-estrone glucuronide conjugates. The resolution of the system allowed the identification of individual conjugate families which differed only in the position of acylation or in the number of estrone glucuronide units. Acid-PAGE was a good alternative to denaturing cation-exchange chromatography for the analysis, separation, and small-scale purification of lysozyme-estrone glucuronide conjugates. It revealed the true order of the relative degree of positive charge on the lysozyme-estrone glucuronide conjugates.
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ISSN:1043-1802
DOI:10.1021/bc9701914