The Potential of Eight Plasma Proteins as Biomarkers in Redefining Leptospirosis Diagnosis

Leptospirosis, a notifiable endemic disease in Malaysia, has higher mortality rates than regional dengue fever. Diverse clinical symptoms and limited diagnostic methods complicate leptospirosis diagnosis. The demand for accurate biomarker-based diagnostics is increasing. This study investigated the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of proteome research Jg. 23; H. 9; S. 4027
Hauptverfasser: Fish-Low, Cheng-Yee, Than, Leslie Thian Lung, Ling, King-Hwa, Sekawi, Zamberi
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 06.09.2024
Schlagworte:
Acute-Phase Proteins - analysis
Adult
Biomarkers - blood
Blood Proteins - analysis
C-Reactive Protein - analysis
Carrier Proteins - blood
Case-Control Studies
Dengue - blood
Dengue - diagnosis
Female
Glycoproteins
Humans
Leptospirosis - blood
Leptospirosis - diagnosis
Male
Membrane Glycoproteins - blood
Membrane Proteins - blood
Middle Aged
Platelet Factor 4 - blood
Proteome - analysis
Proteomics - methods
Serum Amyloid A Protein - analysis
Thrombospondin 1 - blood
iTRAQ
leptospirosis
biomarker
plasma proteome
ISSN:1535-3907, 1535-3907
Online-Zugang:Weitere Angaben
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Leptospirosis, a notifiable endemic disease in Malaysia, has higher mortality rates than regional dengue fever. Diverse clinical symptoms and limited diagnostic methods complicate leptospirosis diagnosis. The demand for accurate biomarker-based diagnostics is increasing. This study investigated the plasma proteome of leptospirosis patients with leptospiraemia and seroconversion compared with dengue patients and healthy subjects using isobaric tags for relative and absolute quantitation (iTRAQ)-mass spectrometry (MS). The iTRAQ analysis identified a total of 450 proteins, which were refined to a list of 290 proteins through a series of exclusion criteria. Differential expression in the plasma proteome of leptospirosis patients compared to the control groups identified 11 proteins, which are apolipoprotein A-II (APOA2), C-reactive protein (CRP), fermitin family homolog 3 (FERMT3), leucine-rich alpha-2-glycoprotein 1 (LRG1), lipopolysaccharide-binding protein (LBP), myosin-9 (MYH9), platelet basic protein (PPBP), platelet factor 4 (PF4), profilin-1 (PFN1), serum amyloid A-1 protein (SAA1), and thrombospondin-1 (THBS1). Following a study on a verification cohort, a panel of eight plasma protein biomarkers was identified for potential leptospirosis diagnosis: CRP, LRG1, LBP, MYH9, PPBP, PF4, SAA1, and THBS1. In conclusion, a panel of eight protein biomarkers offers a promising approach for leptospirosis diagnosis, addressing the limitations of the "one disease, one biomarker" concept.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1535-3907
1535-3907
DOI:10.1021/acs.jproteome.4c00376