Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia.
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| Názov: | Molecular identification of Brucella abortus in secondary hosts of goats and sheep in Magelang, Central Java, Indonesia. |
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| Autori: | Ardiyanto, David1, Wuryastuty, Hastari2 hastari@ugm.ac.id, Yanuartono, Yanuartono2, Sarmin, Sarmin3, Wasito, Raden4, Airin, Claude Mona3, Widiati, Diah Tri5, Mubarokah, Wida Wahidah6 |
| Zdroj: | Open Veterinary Journal. 2025, Vol. 15 Issue 11, p5658-5668. 11p. |
| Druh dokumentu: | Article |
| Predmet: | Brucella abortus, Molecular diagnosis, Polymerase chain reaction, Sheep, Goats, Infectious disease transmission, Phylogeny |
| Geografický termín: | Indonesia, Jawa Tengah (Indonesia) |
| Author-Supplied Keywords: | AMOS PCR Phylogenetic IS711 gene |
| Abstrakt: | Background: Brucella abortus is primarily associated with cattle; however, its detection in small ruminants has been increasingly reported, raising concerns about interspecies transmission and public health risks. Aim: This study aimed to detect and genetically characterize B. abortus in goats and sheep using molecular techniques, assess genetic variation within the IS711 gene, and evaluate phylogenetic relationships among isolates. Methods: Blood samples were collected from 102 small ruminants (32 goats and 70 sheep) with a history of reproductive disorders in Magelang District, Central Java, between October and December 2024. DNA was extracted and analyzed using Abortus, Melitensis, Ovis, Suis Polymerase Chain Reaction (AMOS)-PCR targeting the IS711 gene. Positive amplicons were sequenced and subjected to BLAST analysis, phylogenetic reconstruction (MEGA 12), and genetic distance estimation using the Kimura-2 parameter. Results: AMOS-PCR was used to detect B. abortus Deoxyribonucleic acid in 40 animals (39.2%). Sequencing showed >99% identity with reference B. abortus strains. Phylogenetic analysis revealed low genetic divergence (0.000-0.004), indicating a common infection source. Notably, several isolates exhibited limited sequence variation, including a double guanine insertion at positions 8-9 and a C→T transition at position 40 of IS711. Conclusion: This study demonstrates that B. abortus can infect small ruminants through interspecies transmission under mixed grazing systems. The findings emphasize the need to expand surveillance and control efforts beyond cattle to include sheep and goats, strengthening brucellosis management within a One Health framework. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Doctoral Student of Veterinary Science Study Program, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia 2Department of Veterinary Internal Medicine, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia 3Department of Veterinary Physiology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia 4Department of Veterinary Pathology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia 5Department of Animal Breeding and Reproduction, Faculty of Animal Husbandry, Gadjah Mada University, Yogyakarta, Indonesia 6Agricultural Development Polytechnic, Yogyakarta Magelang, Kabupaten Magelang, Indonesia |
| ISSN: | 2226-4485 |
| DOI: | 10.5455/OVJ.2025.v15.i11.22 |
| Prístupové číslo: | 191964520 |
| Databáza: | Veterinary Source |
| Abstrakt: | Background: Brucella abortus is primarily associated with cattle; however, its detection in small ruminants has been increasingly reported, raising concerns about interspecies transmission and public health risks. Aim: This study aimed to detect and genetically characterize B. abortus in goats and sheep using molecular techniques, assess genetic variation within the IS711 gene, and evaluate phylogenetic relationships among isolates. Methods: Blood samples were collected from 102 small ruminants (32 goats and 70 sheep) with a history of reproductive disorders in Magelang District, Central Java, between October and December 2024. DNA was extracted and analyzed using Abortus, Melitensis, Ovis, Suis Polymerase Chain Reaction (AMOS)-PCR targeting the IS711 gene. Positive amplicons were sequenced and subjected to BLAST analysis, phylogenetic reconstruction (MEGA 12), and genetic distance estimation using the Kimura-2 parameter. Results: AMOS-PCR was used to detect B. abortus Deoxyribonucleic acid in 40 animals (39.2%). Sequencing showed >99% identity with reference B. abortus strains. Phylogenetic analysis revealed low genetic divergence (0.000-0.004), indicating a common infection source. Notably, several isolates exhibited limited sequence variation, including a double guanine insertion at positions 8-9 and a C→T transition at position 40 of IS711. Conclusion: This study demonstrates that B. abortus can infect small ruminants through interspecies transmission under mixed grazing systems. The findings emphasize the need to expand surveillance and control efforts beyond cattle to include sheep and goats, strengthening brucellosis management within a One Health framework. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 22264485 |
| DOI: | 10.5455/OVJ.2025.v15.i11.22 |