Gallibacterium anatis Emerging in Indonesia: Isolation and Molecular Characterization From Chickens in a West Java Poultry Farm.
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| Název: | Gallibacterium anatis Emerging in Indonesia: Isolation and Molecular Characterization From Chickens in a West Java Poultry Farm. |
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| Autoři: | Amaliah, Alya1 (AUTHOR), Mayasari, Ni Luh Putu Ika2 (AUTHOR), Kurnia, Ryan Septa3 (AUTHOR), Nugroho, Christian Marco Hadi3 (AUTHOR), Putra, Muhammad Ade3 (AUTHOR), Indrawati, Agustin2 (AUTHOR) indraseta@apps.ipb.ac.id, Rabbani, Imtiaz (AUTHOR) imtiaz.rabbani@uvas.edu.pk |
| Zdroj: | Veterinary Medicine International. 2/24/2026, Vol. 2026, p1-7. 7p. |
| Druh dokumentu: | Article |
| Témata: | Chickens, Molecular diagnosis, Poultry farms, Indonesians, Microbial virulence genetics, Agricultural egg production, Respiratory diseases, Bacteria |
| Geografický termín: | Jawa Barat (Indonesia), Indonesia |
| Author-Supplied Keywords: | emerging disease Gallibacterium anatis PCR phenotypic characterization poultry |
| Abstrakt: | Gallibacterium anatis (G. anatis) is associated with decreased egg production and respiratory disorders in chickens. The presence of G. anatis in Indonesia has not been reported. This study aimed to identify G. anatis both phenotypically and genotypically in layer, broiler, and breeder chicken from farms in West Java. A total of 23 suspected gallibacteriosis cases were collected from chickens exhibiting respiratory and reproductive disorders between May 2020 and October 2023. Phenotypic characterization was conducted using blood agar culture, Gram staining, and biochemical tests (catalase, oxidase, and TSIA). Genotypic identification of G. anatis was performed using PCR targeting the 16S‐23S rRNA gene, followed by detection of virulence genes (gtxA, flfA, and gyrB). Sequencing and phylogenetic analyses were carried out using BLAST program and MEGA 7. Among the 23 collected samples, 17 samples showed phenotypic characteristics of G. anatis, and 14 of these isolates (73.9%) were confirmed molecularly. Virulence genes profiling revealed the presence of gyrB (100%), gtxA (93.3%), and flfA (71.4%). Sequence analysis demonstrated more than 98.65% similarity between all isolates and reference G. anatis strains in GenBank, supporting accurate species identification. This study provides the molecular evidence of G. anatis in layer, breeder, and broiler chickens from farms in West Java, Indonesia. The detection of key virulence genes further indicates the pathogen's potential role in reduced production and respiratory disease in Indonesian poultry. These findings highlight the importance of routine surveillance and molecular diagnostics to support early detection and control strategies for gallibacteriosis in commercial poultry populations. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Animal Biomedical Sciences,, School of Veterinary Medicine and Biomedical Sciences,, IPB University,, Bogor, 16680,, Indonesia, ipb.ac.id 2Division of Medical Microbiology,, School of Veterinary Medicine and Biomedical Sciences,, IPB University,, Bogor, 16680,, Indonesia, ipb.ac.id 3Animal Health Diagnostic Unit,, PT Medika Satwa Laboratoris,, Bogor, 16166,, Indonesia |
| Full Text Word Count: | 4655 |
| ISSN: | 2090-8113 |
| DOI: | 10.1155/vmi/7717406 |
| Přístupové číslo: | 191893940 |
| Databáze: | Veterinary Source |
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| Abstrakt: | Gallibacterium anatis (G. anatis) is associated with decreased egg production and respiratory disorders in chickens. The presence of G. anatis in Indonesia has not been reported. This study aimed to identify G. anatis both phenotypically and genotypically in layer, broiler, and breeder chicken from farms in West Java. A total of 23 suspected gallibacteriosis cases were collected from chickens exhibiting respiratory and reproductive disorders between May 2020 and October 2023. Phenotypic characterization was conducted using blood agar culture, Gram staining, and biochemical tests (catalase, oxidase, and TSIA). Genotypic identification of G. anatis was performed using PCR targeting the 16S‐23S rRNA gene, followed by detection of virulence genes (gtxA, flfA, and gyrB). Sequencing and phylogenetic analyses were carried out using BLAST program and MEGA 7. Among the 23 collected samples, 17 samples showed phenotypic characteristics of G. anatis, and 14 of these isolates (73.9%) were confirmed molecularly. Virulence genes profiling revealed the presence of gyrB (100%), gtxA (93.3%), and flfA (71.4%). Sequence analysis demonstrated more than 98.65% similarity between all isolates and reference G. anatis strains in GenBank, supporting accurate species identification. This study provides the molecular evidence of G. anatis in layer, breeder, and broiler chickens from farms in West Java, Indonesia. The detection of key virulence genes further indicates the pathogen's potential role in reduced production and respiratory disease in Indonesian poultry. These findings highlight the importance of routine surveillance and molecular diagnostics to support early detection and control strategies for gallibacteriosis in commercial poultry populations. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 20908113 |
| DOI: | 10.1155/vmi/7717406 |