Proteomic signatures of cervical mucus associated with fertility in Bali heifers (Bos javanicus): Implications for biomarker-based selection in artificial insemination programs.
Gespeichert in:
| Titel: | Proteomic signatures of cervical mucus associated with fertility in Bali heifers (Bos javanicus): Implications for biomarker-based selection in artificial insemination programs. |
|---|---|
| Autoren: | Yusuf, Muhammad1 myusuf@unhas.ac.id, Toleng, Abdul Latief1 latief.toleng@yahoo.com, Hasrin, Hasrin2 hasrin@unhas.ac.id, Baharun, Abdullah3 abdullah.baharun@unida.ac.id, Diansyah, Athhar Manabi1 athhar.md@unhas.ac.id, Santoso, Santoso4 sant008@brin.go.id, Rahmat, Rahmat5 rahmatr@ulm.ac.id, Alfian, Andi Muhammad1 alfianam19i@student.unhas.ac.id, Masturi, Masturi1 masturi@unhas.ac.id, Sahiruddin, Sahiruddin1 sahirsabile@unhas.ac.id, Amrullah, Muhammad Fajar6 amrullahfajarmuhammad@apps.ipb.ac.id, Adam, Ahmad Alfaruqi Syahrandi7 adamaas24p@ms.unhas.ac.id, Jannah, Miftahul8 miftahlmiftahul@apps.ipb.ac.id |
| Quelle: | Veterinary World. Jan2026, Vol. 19 Issue 1, p135-148. 14p. |
| Publikationsart: | Article |
| Schlagworte: | Fertility, Artificial insemination, Protein analysis, Biomarkers, Sperm-ovum interactions, Proteomics, Body fluids, Cattle |
| Geografische Kategorien: | Bali Island (Indonesia), Indonesia |
| Author-Supplied Keywords: | artificial insemination Bali cattle biomarkers cervical mucus fertility heifers proteomics reproductive efficiency |
| Abstract: | Background and Aim: Despite strong adaptive traits, the reproductive efficiency of Bali cattle (Bos javanicus) remains suboptimal, with low conception rates following artificial insemination (AI). Cervical mucus (CM) is a critical factor in sperm transport and fertilization; however, its molecular basis in relation to fertility has not been elucidated in this indigenous breed. This study aimed to characterize the proteomic profile of CM in Bali heifers and to identify protein biomarkers associated with fertility-related mucus quality. Materials and Methods: The study was conducted between February and August 2024 in South Sulawesi, Indonesia. Forty clinically healthy Bali heifers (2-3 years old) were sampled during natural oestrus and divided into good CM (GCM; n = 20) and poor CM (PCM; n = 20) groups using a validated five-parameter biophysical scoring system. CM proteins were extracted and analyzed using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by liquid chromatography-tandem mass spectrometry. High-confidence protein identification was achieved at <1% false discovery rate, and differential abundance was evaluated using Benjamini-Hochberg correction (p < 0.05). Functional enrichment, correlation analysis with mucus traits, and receiver-operating-characteristic (ROC) analyses with cross-validation were performed. Results: Significant differences (p < 0.05) were observed between GCM and PCM groups for appearance, viscosity, spinnbarkeit, and ferning pattern, while pH did not differ. A total of 52 proteins were identified after quality control, of which 13 showed significant differential abundance. GCM was characterized by higher levels of NT5E, lactoferrin, SCGB1D, and lactotransferrin, whereas PCM showed enrichment of complement factor I (CFI), haptoglobin (HP), MUC5AC, FAIM2, TIMP2, PEBP4, SAA3, GRP, and IGL. Functional enrichment analysis indicated anti-inflammatory and epithelial-protective pathways in GCM, in contrast to complement activation, proteolysis, and oxidative remodeling in PCM. ROC analysis demonstrated excellent discriminative performance for NT5E (GCM) and CFI and haptoglobin (PCM), each achieving an area under the curve of 1.00 in this cohort. Conclusion: This study offers the first proteomic evidence connecting CM composition to fertility-related traits in Bali heifers. NT5E, CFI, and HP stand out as promising biomarkers for fertility screening, providing a molecular framework to improve AI efficiency and selection strategies in indigenous cattle. [ABSTRACT FROM AUTHOR] |
| Copyright of Veterinary World is the property of Veterinary World and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Author Affiliations: | 1Faculty of Animal Science, Hasanuddin University, Jl. Perintis Kemeredekaan Km. 10 Tamalanrea Makassar, South Sulawesi, Indonesia 2Faculty of Vocation, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10 Tamalanrea Makassar, South Sulawesi, Indonesia 3Faculty of Agriculture, Djuanda University, Jl. Tol Ciawi No. 1, Ciawi, West Java, Indonesia 4Research Center for Animal Husbandry, National Research and Innovation Agency, Cibinong Science Center, Bogor, Indonesia 5Faculty of Agriculture, Lambung Mangkurat University, Jl. Jenderal Ahmad Yani Km. 36, Banjarbaru, South Kalimantan, 70714, Indonesia 6Doctoral Program of Animal Biomedical Science, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, West Java, Indonesia 7Graduate School, Hasanuddin University, Jl. Perintis Kemeredekaan Km. 10 Tamalanrea Makassar, South Sulawesi, Indonesia 8Master Program of Animal Biomedical Science, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, 16680, West Java, Indonesia |
| ISSN: | 0972-8988 |
| DOI: | 10.14202/vetworld.2026.135-148 |
| Dokumentencode: | 191316071 |
| Datenbank: | Veterinary Source |
| Abstract: | Background and Aim: Despite strong adaptive traits, the reproductive efficiency of Bali cattle (Bos javanicus) remains suboptimal, with low conception rates following artificial insemination (AI). Cervical mucus (CM) is a critical factor in sperm transport and fertilization; however, its molecular basis in relation to fertility has not been elucidated in this indigenous breed. This study aimed to characterize the proteomic profile of CM in Bali heifers and to identify protein biomarkers associated with fertility-related mucus quality. Materials and Methods: The study was conducted between February and August 2024 in South Sulawesi, Indonesia. Forty clinically healthy Bali heifers (2-3 years old) were sampled during natural oestrus and divided into good CM (GCM; n = 20) and poor CM (PCM; n = 20) groups using a validated five-parameter biophysical scoring system. CM proteins were extracted and analyzed using one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by liquid chromatography-tandem mass spectrometry. High-confidence protein identification was achieved at <1% false discovery rate, and differential abundance was evaluated using Benjamini-Hochberg correction (p < 0.05). Functional enrichment, correlation analysis with mucus traits, and receiver-operating-characteristic (ROC) analyses with cross-validation were performed. Results: Significant differences (p < 0.05) were observed between GCM and PCM groups for appearance, viscosity, spinnbarkeit, and ferning pattern, while pH did not differ. A total of 52 proteins were identified after quality control, of which 13 showed significant differential abundance. GCM was characterized by higher levels of NT5E, lactoferrin, SCGB1D, and lactotransferrin, whereas PCM showed enrichment of complement factor I (CFI), haptoglobin (HP), MUC5AC, FAIM2, TIMP2, PEBP4, SAA3, GRP, and IGL. Functional enrichment analysis indicated anti-inflammatory and epithelial-protective pathways in GCM, in contrast to complement activation, proteolysis, and oxidative remodeling in PCM. ROC analysis demonstrated excellent discriminative performance for NT5E (GCM) and CFI and haptoglobin (PCM), each achieving an area under the curve of 1.00 in this cohort. Conclusion: This study offers the first proteomic evidence connecting CM composition to fertility-related traits in Bali heifers. NT5E, CFI, and HP stand out as promising biomarkers for fertility screening, providing a molecular framework to improve AI efficiency and selection strategies in indigenous cattle. [ABSTRACT FROM AUTHOR] |
|---|---|
| ISSN: | 09728988 |
| DOI: | 10.14202/vetworld.2026.135-148 |