Cross-domain antimicrobial resistance in poultry farming: A One Health assessment of antimicrobial use and multidrug resistance in Kiambu County, Kenya.
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| Názov: | Cross-domain antimicrobial resistance in poultry farming: A One Health assessment of antimicrobial use and multidrug resistance in Kiambu County, Kenya. |
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| Autori: | Munene, Ann Kangai1,2 annkmunene@gmail.com, Mwangi, Peter Muiruri3 muirurimwangip@gmail.com, Bebora, Lilly Caroline2 bebora@uonbi.ac.ke, Mbindyo, Christine Minoo2 cminoo@uonbi.ac.ke, Maingi, John Muthini1 maingi.muthini@ku.ac.ke |
| Zdroj: | Veterinary World. Jan2026, Vol. 19 Issue 1, p1-14. 14p. |
| Druh dokumentu: | Article |
| Author-Supplied Keywords: | antimicrobial resistance Antimicrobial use Enterococcus Escherichia coli Kenya One Health poultry farming |
| Abstrakt: | Background and Aim: Antimicrobial resistance (AMR) has emerged as a major One Health threat driven by inappropriate antimicrobial use (AMU) in humans, animals, and the environment. Poultry production is recognized as a key reservoir of antimicrobial-resistant bacteria, yet few studies in Kenya examine AMU and AMR across interconnected human-animal-environment domains. This study assessed AMU patterns among poultry farmers in Kiambu County and characterized phenotypic resistance in Escherichia coli and Enterococcus spp. isolated from humans, chickens, and chicken environments. Materials and Methods: A cross-sectional study was conducted from June to September 2024, involving 102 poultry farms. Farm demographics and AMU data were collected using a semi-structured questionnaire. Archived E. coli (n = 92) and Enterococcus spp. (n = 101) isolates from chicken handlers' hands, chickens, and environmental samples were subjected to antimicrobial susceptibility testing using the Kirby-Bauer method per Clinical and Laboratory Standards Institute (CLSI) 2024 guidelines. Descriptive and inferential statistics, including logistic regression with false discovery rate correction, were used to assess associations between AMU and phenotypic resistance. Results: Macrolides (69%), tetracyclines (48%), and sulfonamides (21%) were the most commonly used antimicrobials; 7% of farms reported colistin use. Among E. coli isolates, resistance was highest to ampicillin (77%), tetracycline (72%), and trimethoprim-sulfamethoxazole (49%), with 35% exhibiting multidrug resistance (MDR). No carbapenem resistance was detected. Enterococcus isolates showed high erythromycin resistance (61%) and moderate ciprofloxacin resistance (26%), with 6.9% exhibiting MDR; no vancomycin-resistant enterococci (VRE) were observed. Penicillin use strongly predicted ampicillin resistance in both organisms, whereas sulfonamide use was associated with reduced trimethoprim-sulfamethoxazole resistance. Macrolide use did not correlate with erythromycin resistance. Conclusion: High AMU in poultry farming, particularly of macrolides, tetracyclines, and sulfonamides, has created significant selection pressure, contributing to MDR emergence across One Health interfaces. Detection of resistance in humans, poultry, and shared environments underscores the bidirectional risk of AMR transmission. Strengthened antimicrobial stewardship, regulation of critically important antimicrobials, and enhanced farm hygiene are essential to mitigate AMR. These findings directly support Kenya's Vision 2030 and SDGs targeting health, responsible production, and environmental protection. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Department of Biochemistry, Microbiology, and Biotechnology, Kenyatta University, Nairobi, Kenya 2Department of Veterinary Pathology, Microbiology, and Parasitology, University of Nairobi, Nairobi, Kenya 3International Center of Insect Physiology and Ecology, Nairobi, Kenya |
| ISSN: | 0972-8988 |
| DOI: | 10.14202/vetworld.2026.1-14 |
| Prístupové číslo: | 191316061 |
| Databáza: | Veterinary Source |
| Abstrakt: | Background and Aim: Antimicrobial resistance (AMR) has emerged as a major One Health threat driven by inappropriate antimicrobial use (AMU) in humans, animals, and the environment. Poultry production is recognized as a key reservoir of antimicrobial-resistant bacteria, yet few studies in Kenya examine AMU and AMR across interconnected human-animal-environment domains. This study assessed AMU patterns among poultry farmers in Kiambu County and characterized phenotypic resistance in Escherichia coli and Enterococcus spp. isolated from humans, chickens, and chicken environments. Materials and Methods: A cross-sectional study was conducted from June to September 2024, involving 102 poultry farms. Farm demographics and AMU data were collected using a semi-structured questionnaire. Archived E. coli (n = 92) and Enterococcus spp. (n = 101) isolates from chicken handlers' hands, chickens, and environmental samples were subjected to antimicrobial susceptibility testing using the Kirby-Bauer method per Clinical and Laboratory Standards Institute (CLSI) 2024 guidelines. Descriptive and inferential statistics, including logistic regression with false discovery rate correction, were used to assess associations between AMU and phenotypic resistance. Results: Macrolides (69%), tetracyclines (48%), and sulfonamides (21%) were the most commonly used antimicrobials; 7% of farms reported colistin use. Among E. coli isolates, resistance was highest to ampicillin (77%), tetracycline (72%), and trimethoprim-sulfamethoxazole (49%), with 35% exhibiting multidrug resistance (MDR). No carbapenem resistance was detected. Enterococcus isolates showed high erythromycin resistance (61%) and moderate ciprofloxacin resistance (26%), with 6.9% exhibiting MDR; no vancomycin-resistant enterococci (VRE) were observed. Penicillin use strongly predicted ampicillin resistance in both organisms, whereas sulfonamide use was associated with reduced trimethoprim-sulfamethoxazole resistance. Macrolide use did not correlate with erythromycin resistance. Conclusion: High AMU in poultry farming, particularly of macrolides, tetracyclines, and sulfonamides, has created significant selection pressure, contributing to MDR emergence across One Health interfaces. Detection of resistance in humans, poultry, and shared environments underscores the bidirectional risk of AMR transmission. Strengthened antimicrobial stewardship, regulation of critically important antimicrobials, and enhanced farm hygiene are essential to mitigate AMR. These findings directly support Kenya's Vision 2030 and SDGs targeting health, responsible production, and environmental protection. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 09728988 |
| DOI: | 10.14202/vetworld.2026.1-14 |