Silico study: Hepatoprotective effects of capsaicin on the liver of aflatoxin B1-intoxicated mice (Mus musculus).
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| Title: | Silico study: Hepatoprotective effects of capsaicin on the liver of aflatoxin B1-intoxicated mice (Mus musculus). |
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| Authors: | Sukmanadi, Mohammad1, Sudjarwo, Sri Agus1, Effendi, Mustofa Helmi2, Khairullah, Aswin Rafif3, Madyawati, Sri Pantja4, Mustofa, Imam4, Srianto, Pudji4, Lamid, Mirni5, Plumeriastuti, Hani6, Ahmad, Riza Zainuddin3, Akintunde, Adeyinka Oye7, Awwanah, Mo8, Pratama, Bima Putra9, Prasetyo, Agung10 |
| Source: | Open Veterinary Journal. 2025, Vol. 15 Issue 10, p5335-5345. 11p. |
| Document Type: | Article |
| Subjects: | Capsaicin, Aflatoxins, Bioinformatics, Mitogen-activated protein kinases, Molecular docking, Liver regeneration, Mice, Protein kinase B |
| Author-Supplied Keywords: | Aflatoxin B1 Good health and wellbeing Hepatoprotection Herbal medicine |
| Abstract: | Background: Mycotoxins are secondary metabolites produced by fungi that cause serious diseases and death. Among the mycotoxins, aflatoxins stand out for their significant toxic impact, especially Aflatoxin B1 (AFB1) produced by Aspergillus species. Capsaicin, known for its pungent taste, is also highlighted in this study for its potential health benefits, which have been shown to have beneficial effects on the cardiovascular system, metabolism, analgesia, and management of disorders. Aim: This study aimed to determine the in silico inhibition effect of capsaicin on the expression of AKT1 and MAPK1 target proteins in mice due to AFB1 induction. Methods: Molecular docking is a bioinformatics technique that combines biological knowledge with databases (big data) to ascertain the pattern, affinity, and possible activity of chemical compounds interacting with target proteins. When the Gibbs free energy (ΔG) changes, protein-ligand interaction occurs. Results: The findings demonstrated that molecular docking, protein-ligand interactions, homology modeling, target protein prediction, protein interaction analysis, and molecular visualization in silico. The target proteins AKT1 and MAPK1 were highly expressed in the AFB1 binding prediction results in mice (Mus musculus). The tethering of negative Gibbs free energy molecules was deemed satisfactory, and 3D virtual visualization demonstrated excellent adhesion to the receptors of the molecules. Conclusion: Capsaicin can synergistically inhibit regulatory cells through the AKT 1 and MAPK 1 signaling pathways, potentiating the hepatotoxicity action protective effect of capsaicin against AFB-1 intoxication. Therefore, capsaicin is a potential candidate for herbal medicine as a hepatoprotective agent. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 2Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 3Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia. 4Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 5Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 6Division of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 7Department of Agriculture and Industrial Technology, Babcock University, Ilishan Remo, Nigeria. 8Research Center for Applied Botany, National Research and Innovation Agency (BRIN), Bogor, Indonesia. 9Research Center for Agroindustry, National Research and Innovation Agency (BRIN), South Tangerang, Indonesia. 10Research Center for Estate Crops, National Research and Innovation Agency (BRIN), Bogor, Indonesia. |
| ISSN: | 2226-4485 |
| DOI: | 10.5455/OVJ.2025.v15.i10.50 |
| Accession Number: | 190365254 |
| Database: | Veterinary Source |
| Abstract: | Background: Mycotoxins are secondary metabolites produced by fungi that cause serious diseases and death. Among the mycotoxins, aflatoxins stand out for their significant toxic impact, especially Aflatoxin B1 (AFB1) produced by Aspergillus species. Capsaicin, known for its pungent taste, is also highlighted in this study for its potential health benefits, which have been shown to have beneficial effects on the cardiovascular system, metabolism, analgesia, and management of disorders. Aim: This study aimed to determine the in silico inhibition effect of capsaicin on the expression of AKT1 and MAPK1 target proteins in mice due to AFB1 induction. Methods: Molecular docking is a bioinformatics technique that combines biological knowledge with databases (big data) to ascertain the pattern, affinity, and possible activity of chemical compounds interacting with target proteins. When the Gibbs free energy (ΔG) changes, protein-ligand interaction occurs. Results: The findings demonstrated that molecular docking, protein-ligand interactions, homology modeling, target protein prediction, protein interaction analysis, and molecular visualization in silico. The target proteins AKT1 and MAPK1 were highly expressed in the AFB1 binding prediction results in mice (Mus musculus). The tethering of negative Gibbs free energy molecules was deemed satisfactory, and 3D virtual visualization demonstrated excellent adhesion to the receptors of the molecules. Conclusion: Capsaicin can synergistically inhibit regulatory cells through the AKT 1 and MAPK 1 signaling pathways, potentiating the hepatotoxicity action protective effect of capsaicin against AFB-1 intoxication. Therefore, capsaicin is a potential candidate for herbal medicine as a hepatoprotective agent. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 22264485 |
| DOI: | 10.5455/OVJ.2025.v15.i10.50 |