Molecular Detection and Evolutionary Insights of Contagious Ecthyma (Orf) Virus in Goat in Andhra Pradesh, India.
Gespeichert in:
| Titel: | Molecular Detection and Evolutionary Insights of Contagious Ecthyma (Orf) Virus in Goat in Andhra Pradesh, India. |
|---|---|
| Autoren: | Bodlapati, Vimala Devi1 bvimala96@gmail.com, Gangaraju, Sireesha1, Dwarakacherla, Neeraja1, Narayana, Ramachandra2, Gundampati, Ravi babu3 |
| Quelle: | Indian Journal of Veterinary Sciences & Biotechnology. Sep/Oct2025, Vol. 21 Issue 5, p98-103. 6p. |
| Publikationsart: | Article |
| Schlagworte: | Goats, Virus diseases, Gene mapping, Zoonoses, Vaccine development, Phylogeny |
| Geografische Kategorien: | Andhra Pradesh (India), India |
| Author-Supplied Keywords: | Capripox viruses Contagious ecthyma GM-CSF/IL2 inhibitor gene Orf virus (ORFV) Phylogenetic analysis |
| Abstract: | Contagious ecthyma or Orf is a zoonotic, transboundary disease affecting both domestic and wild ruminants. The present study investigated the incidence of severe Orf outbreaks in goats in Kurnool and Anantapur districts of Andhra Pradesh, a southern state of India during 2024. The presence of the Orf virus in the clinical samples was confirmed by PCR targeting the GM-CSF/IL-2 inhibitor (GIF) gene. Among the 11 samples tested, four skin scab samples yielded a positive result, showing a specific 408 bp PCR amplicon. Further testing using a capripox genus-specific PCR assay confirmed the absence of sheep pox and goat pox viruses in the samples. This is the first study on comprehensive genetic characterization of circulating ORFV strains in Andhra Pradesh. Phylogenetic analysis revealed two ORFV isolates (ATP 2833 and KRNL 4410) belong to distinct groups, sharing 97.46% nucleotide identity with each other. ATP 2833 isolate showed highest homology with Tamil Nadu field isolates and the TANUVAS P15 vaccine strain suggesting southern Indian ORFV circulation patterns. In contrast, KRNL 4410 strain demonstrated close phylogenetic proximity to the IVRI Mukteshwar P50 vaccine strain and Ludhiana 2006 strain, suggesting a possible association with vaccine-related lineages circulating in northern India. The divergence of both strains from the first-generation NZ2 isolate (1982) and the Japan (1985) isolate highlights significant evolutionary changes over time. These alterations are likely driven by regional transmission dynamics such as climate, host and breed characteristics. These results indicate the potential for regional variations in ORFV strains, which could have important implications for vaccine development and the optimization of disease control strategies. [ABSTRACT FROM AUTHOR] |
| Copyright of Indian Journal of Veterinary Sciences & Biotechnology is the property of Indian Journal of Veterinary Sciences & Biotechnology and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Author Affiliations: | 1Veterinary Biological & Research Institute (VBRI), Labbipet-520010, Vijayawada, Andhra Pradesh, India 2Animal Disease Diagnostic Laboratory (ADDL), Anantapur-515001, Andhra Pradesh, India 3Animal Disease Diagnostic Laboratory (ADDL), Kurnool-518001, Andhra Pradesh, India |
| ISSN: | 2394-0247 |
| DOI: | 10.48165/ijvsbt.21.5.19 |
| Dokumentencode: | 189725766 |
| Datenbank: | Veterinary Source |
| Abstract: | Contagious ecthyma or Orf is a zoonotic, transboundary disease affecting both domestic and wild ruminants. The present study investigated the incidence of severe Orf outbreaks in goats in Kurnool and Anantapur districts of Andhra Pradesh, a southern state of India during 2024. The presence of the Orf virus in the clinical samples was confirmed by PCR targeting the GM-CSF/IL-2 inhibitor (GIF) gene. Among the 11 samples tested, four skin scab samples yielded a positive result, showing a specific 408 bp PCR amplicon. Further testing using a capripox genus-specific PCR assay confirmed the absence of sheep pox and goat pox viruses in the samples. This is the first study on comprehensive genetic characterization of circulating ORFV strains in Andhra Pradesh. Phylogenetic analysis revealed two ORFV isolates (ATP 2833 and KRNL 4410) belong to distinct groups, sharing 97.46% nucleotide identity with each other. ATP 2833 isolate showed highest homology with Tamil Nadu field isolates and the TANUVAS P15 vaccine strain suggesting southern Indian ORFV circulation patterns. In contrast, KRNL 4410 strain demonstrated close phylogenetic proximity to the IVRI Mukteshwar P50 vaccine strain and Ludhiana 2006 strain, suggesting a possible association with vaccine-related lineages circulating in northern India. The divergence of both strains from the first-generation NZ2 isolate (1982) and the Japan (1985) isolate highlights significant evolutionary changes over time. These alterations are likely driven by regional transmission dynamics such as climate, host and breed characteristics. These results indicate the potential for regional variations in ORFV strains, which could have important implications for vaccine development and the optimization of disease control strategies. [ABSTRACT FROM AUTHOR] |
|---|---|
| ISSN: | 23940247 |
| DOI: | 10.48165/ijvsbt.21.5.19 |