IMMUNOPATHOLOGICAL EVALUATION OF GREEN SYNTHESIZED (ZINC OXIDE NANOPARTICLE OF AQUEOUS FRACTA) EXTRACT AS A TREATMENT OF ULCERATIVE COLITIS IN RATS.
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| Title: | IMMUNOPATHOLOGICAL EVALUATION OF GREEN SYNTHESIZED (ZINC OXIDE NANOPARTICLE OF AQUEOUS FRACTA) EXTRACT AS A TREATMENT OF ULCERATIVE COLITIS IN RATS. |
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| Authors: | FARIS, MARIWAN KAMIL1 mk230024pve@st.tu.edu.iq, Al-BAYAT, HASSAN H. K.2 dr_patho80@tu.edu.iq, SAEED, HUSAMULDEEN S. M.3 Sbc.s4@tu.edu.iq |
| Source: | Assiut Veterinary Medical Journal. Oct2025, Vol. 71 Issue 187, p605-616. 12p. |
| Document Type: | Article |
| Subjects: | Ulcerative colitis, Immune response, Herbal medicine, Rats, Inflammatory mediators, Zinc oxide, Biosynthesis, Pathological physiology |
| Author-Supplied Keywords: | Histopathological finding IL 1 β IL-6 TNF-α |
| Abstract (English): | Interest in exploring natural substances as alternative treatments for bowel diseases continues to grow. Although green synthesis has repeatedly been mentioned in stimulating both arms of the immune system, this has led to the attraction of inflammatory cells for healing colitis. This study aimed to assess the impact of green-synthesized Zinc Oxide nanoparticles and aqueous extract on enhancing immune and pathological defense mechanisms against colitis in rats. Sixty adult male rats were categorized into six groups at random, (G1) Acetic acid (4%) (1.5 ml/intrarectally(, (G2) normal saline, (G3) Prosopis farcta hydrochloric extract (400 mg/kg/orally), (G4) ZnO-NP of Prosopis farcta (400 mg/kg/orally), (G5) ZnO-NP of Prosopis farcta 400 mg/kg/ orally, in combination with sulfasalazine 100 mg/Kg/orally) and (G6) sulfasalazine group. At the end of the experimental period (20 days), serum was taken for inflammatory markers (TNF-α, IL-1β and IL-6 levels), and the colon tissues were examined histologically. Results showed elevated serum levels of immunological biomarkers between treated and untreated groups. These indicators were observed in the (G1) group. The green synthesized group showed reductions in pro-inflammatory cytokines TNF-α (15.148), IL1β (4.02) and IL-6 (7.88), compared with (G1) (79.968), (31.4) and (30.2), respectively. The enhanced immune response related to colon inflammation is improved with these effects, leading to the disappearance of many pathological lesions. This study revealed that the use of green-synthesized supplemented diets, especially in (400 mg/kg), could improve immune responses and protect the colon and reduce symptoms of gastrointestinal diseases, such as ulcerative colitis. [ABSTRACT FROM AUTHOR] |
| Abstract (Arabic): | المقال يركز على التقييم المناعي المرضي لجزيئات أكسيد الزنك التي تم تصنيعها بطريقة صديقة للبيئة من المستخلص المائي لنبات *Prosopis farcta* كعلاج لالتهاب القولون التقرحي في الفئران. شملت الدراسة ستين فأراً ذكراً تم تقسيمهم إلى ست مجموعات، مع علاجات تشمل حمض الأسيتيك لتحفيز التهاب القولون، وجرعات مختلفة من مستخلص *Prosopis farcta*، وتركيبات مع السلفاسالازين. أظهرت النتائج أن الجزيئات المصنعة بطريقة صديقة للبيئة قد خفضت بشكل كبير من السيتوكينات المؤيدة للالتهابات (TNF-α، IL-1β، وIL-6) مقارنة بالمجموعات غير المعالجة، مما يشير إلى فوائد محتملة في تعزيز الاستجابات المناعية وتخفيف أعراض الأمراض المعوية مثل التهاب القولون التقرحي. تدعم النتائج استخدام المواد الطبيعية كعلاجات بديلة لأمراض الأمعاء. [Extracted from the article] |
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| Author Affiliations: | 1Department of Pharmacology, Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Tikrit. Iraq 2Department of Pathology and Poultry Diseases, Faculty of Veterinary Medicine, University of Tikrit, Iraq 3Department of Pharmacology, Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Tikrit, Iraq |
| ISSN: | 1012-5973 |
| DOI: | 10.21608/avmj.2025.392994.1757 |
| Accession Number: | 189181027 |
| Database: | Veterinary Source |
| Abstract: | Interest in exploring natural substances as alternative treatments for bowel diseases continues to grow. Although green synthesis has repeatedly been mentioned in stimulating both arms of the immune system, this has led to the attraction of inflammatory cells for healing colitis. This study aimed to assess the impact of green-synthesized Zinc Oxide nanoparticles and aqueous extract on enhancing immune and pathological defense mechanisms against colitis in rats. Sixty adult male rats were categorized into six groups at random, (G1) Acetic acid (4%) (1.5 ml/intrarectally(, (G2) normal saline, (G3) Prosopis farcta hydrochloric extract (400 mg/kg/orally), (G4) ZnO-NP of Prosopis farcta (400 mg/kg/orally), (G5) ZnO-NP of Prosopis farcta 400 mg/kg/ orally, in combination with sulfasalazine 100 mg/Kg/orally) and (G6) sulfasalazine group. At the end of the experimental period (20 days), serum was taken for inflammatory markers (TNF-α, IL-1β and IL-6 levels), and the colon tissues were examined histologically. Results showed elevated serum levels of immunological biomarkers between treated and untreated groups. These indicators were observed in the (G1) group. The green synthesized group showed reductions in pro-inflammatory cytokines TNF-α (15.148), IL1β (4.02) and IL-6 (7.88), compared with (G1) (79.968), (31.4) and (30.2), respectively. The enhanced immune response related to colon inflammation is improved with these effects, leading to the disappearance of many pathological lesions. This study revealed that the use of green-synthesized supplemented diets, especially in (400 mg/kg), could improve immune responses and protect the colon and reduce symptoms of gastrointestinal diseases, such as ulcerative colitis. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 10125973 |
| DOI: | 10.21608/avmj.2025.392994.1757 |