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Comparative genomic analysis of multiple mammary tumors from a single dog: whole-genome sequencing study.

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Titel: Comparative genomic analysis of multiple mammary tumors from a single dog: whole-genome sequencing study.
Autoren: Kim, Keon1 (AUTHOR), Shin, Tae-Hoon2 (AUTHOR), Park, Sin-Wook1 (AUTHOR), Park, Sang-Ik3 (AUTHOR), Do, Yoon Jung4 (AUTHOR), Ro, Woong-Bin1,5 (AUTHOR) woongbinro@jnu.ac.kr, Lee, Chang-Min1,5 (AUTHOR) cmlee1122@jnu.ac.kr
Quelle: Irish Veterinary Journal. 10/30/2025, Vol. 78 Issue 1, p1-16. 16p.
Publikationsart: Case Study
Schlagworte: Mammary gland cancer, Whole genome sequencing, Dog genetics, Somatic mutation, Clone cells, Genetic variation, Comparative genomics, Benign tumors
Author-Supplied Keywords: Breast cancer
Canine
Mammary gland tumor
Medical and Health Sciences Oncology and Carcinogenesis Biological Sciences Genetics
Next-generation sequencing
Whole-genome sequencing
Abstract: Background: Next-generation sequencing of canine spontaneous cancer is a powerful approach in both comparative oncology and veterinary genomics. We encountered a unique case with concurrent mammary tumors. Using whole-genome sequencing (WGS), we profiled the tumor-specific landscape of somatic mutations across multiple tumor subtypes, providing unprecedented evidence within an identical genetic background. Results: Of the seven mammary gland tumors (MGTs) isolated, two were diagnosed as benign and five as malignant. High-quality WGS (34.5X average sequencing depth, ≥ 20X coverage across 94.9% of the genome) on tumors and a blood sample revealed missense mutations in human breast cancer-related genes such as BRCA2 and TP53. Furthermore, we found evidence of canine-specific somatic mutations depending on the tumor subtypes, including HECTD4 in malignant tumors and NIPBL in epithelial-derived malignant tumors. Conclusions: This unique case study provides novel insights into the genomic heterogeneity, clonal evolution, and subtype-specific pathogenesis of naturally occurring canine MGTs. Despite some inherent limitations and potential for individual-specific variation, our results emphasize and guide future large-scale, longitudinal studies to further elucidate the clinical and biological significance of key somatic alterations. [ABSTRACT FROM AUTHOR]
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Author Affiliations: 1https://ror.org/05kzjxq56 Department of Veterinary Internal Medicine, College of Veterinary Medicine and BK21 FOUR program, Chonnam National University, 61186, Gwangju, Republic of Korea
2https://ror.org/05hnb4n85 Department of Laboratory Animal Medicine, College of Veterinary Medicine, Veterinary Medical Research Institute, Jeju National University, 63243, Jeju, Republic of Korea
3https://ror.org/05kzjxq56 Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, 61186, Gwangju, Republic of Korea
4https://ror.org/02ty3a980 Division of Animal Diseases & Health, Rural Development Administration, National Institute of Animal Science, 55365, Wanju-gun, Republic of Korea
577 Yongbongro buk-gu, Gwangju, Republic of Korea
Full Text Word Count: 10481
ISSN: 0368-0762
DOI: 10.1186/s13620-025-00311-5
Dokumentencode: 189002670
Datenbank: Veterinary Source
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Beschreibung
Abstract:Background: Next-generation sequencing of canine spontaneous cancer is a powerful approach in both comparative oncology and veterinary genomics. We encountered a unique case with concurrent mammary tumors. Using whole-genome sequencing (WGS), we profiled the tumor-specific landscape of somatic mutations across multiple tumor subtypes, providing unprecedented evidence within an identical genetic background. Results: Of the seven mammary gland tumors (MGTs) isolated, two were diagnosed as benign and five as malignant. High-quality WGS (34.5X average sequencing depth, ≥ 20X coverage across 94.9% of the genome) on tumors and a blood sample revealed missense mutations in human breast cancer-related genes such as BRCA2 and TP53. Furthermore, we found evidence of canine-specific somatic mutations depending on the tumor subtypes, including HECTD4 in malignant tumors and NIPBL in epithelial-derived malignant tumors. Conclusions: This unique case study provides novel insights into the genomic heterogeneity, clonal evolution, and subtype-specific pathogenesis of naturally occurring canine MGTs. Despite some inherent limitations and potential for individual-specific variation, our results emphasize and guide future large-scale, longitudinal studies to further elucidate the clinical and biological significance of key somatic alterations. [ABSTRACT FROM AUTHOR]
ISSN:03680762
DOI:10.1186/s13620-025-00311-5