Comparative virulence study of locally circulating peste des petits ruminants virus (PPRV) strains in indigenous Ethiopian goats.
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| Title: | Comparative virulence study of locally circulating peste des petits ruminants virus (PPRV) strains in indigenous Ethiopian goats. |
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| Authors: | Aklilu, Fasil1,2 (AUTHOR) fasil.aklilu@yahoo.com, Ashenafi, Hagos1 (AUTHOR), Kassa, Tesfu1 (AUTHOR), Chaka, Hassen3 (AUTHOR), Sibhatu, Demeke2 (AUTHOR), Shegu, Dereje2 (AUTHOR), Mohammed, Abde Aliy2 (AUTHOR), Belaineh, Redeat2 (AUTHOR), Kidane, Menbere2 (AUTHOR), Asgedom, Hagos2 (AUTHOR), Chibssa, Tesfaye2 (AUTHOR), Mekonnen, Getnet2 (AUTHOR), Sirak, Asegedetch2 (AUTHOR), Gebredufe, Solomon2 (AUTHOR), Byukusenge, Maurice4 (AUTHOR), Herzog, Catherine M.5 (AUTHOR) catherine.herzog@gmail.com, Kapur, Vivek5,6 (AUTHOR) vkapur@psu.edu |
| Source: | BMC Veterinary Research. 10/6/2025, Vol. 21 Issue 1, p1-11. 11p. |
| Document Type: | Article |
| Subjects: | Peste des petits ruminants, Microbial virulence, Symptoms, Goats, Comparative studies, Seroconversion, Communicable disease control |
| Geographic Terms: | Ethiopia |
| Author-Supplied Keywords: | Comparative virulence Experimental infection Indigenous Goats Pathogenesis PPRV Strain characterization |
| Abstract: | Background: Peste des Petits Ruminants (PPR) is a highly contagious viral disease affecting small ruminants with considerable socioeconomic impacts in Ethiopia. Despite its importance, comparative virulence of locally circulating PPR virus strains in indigenous goats remains poorly characterized. This study evaluated six distinct PPRV strains (four lineage-IV, two lineage-III) in experimentally infected Ethiopian goats. Twenty-one Central highland breed goats (12–30 months) were randomly allocated into six experimental groups and one control group, with three goats in each group and experimental animals inoculated intranasally (104.5 TCID50 per ml). Clinical, serological, molecular, and pathogenesis data were collected. Results: Five of six strains produced consistent clinical manifestations beginning at 4 days post-infection (dpi), with seroconversion by 7 dpi and viral antigens detectable in nasal swabs from 3 dpi. No substantial lineage-specific differences in virulence were observed between lineage-III and lineage-IV strains. One lineage-IV isolate (38,920/19) produced significantly milder clinical manifestations (P < 0.05) and no seroconversion, suggesting potential strain-level variation. Gross and histopathological findings were consistent across virulent strains, with nasal swabs proving most reliable for early diagnosis. Conclusions: This first comparative study of Ethiopian PPRV strains establishes similar virulence profiles across lineages in local goats, suggesting that lineage classification alone may not predict clinical outcomes—an important finding for regional disease control strategies. The reduced virulence in one strain suggests factors beyond genomic lineage may influence experimental outcomes, though additional work with standardized protocols is needed to confirm these findings. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1https://ror.org/038b8e254 Aklilu Lemma Institute of Health Research, Addis Ababa University, Addis Ababa, Ethiopia 2Animal Health Institute, Sebeta, Ethiopia 3Food and Agricultural Organization (NSAH-CJW), Addis Ababa, Ethiopia 4https://ror.org/04p491231 Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, USA 5https://ror.org/04p491231 Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA 6https://ror.org/04p491231 Department of Animal Science, The Pennsylvania State University, University Park, PA, USA |
| Full Text Word Count: | 6928 |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-025-05073-4 |
| Accession Number: | 188712384 |
| Database: | Veterinary Source |
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| Abstract: | Background: Peste des Petits Ruminants (PPR) is a highly contagious viral disease affecting small ruminants with considerable socioeconomic impacts in Ethiopia. Despite its importance, comparative virulence of locally circulating PPR virus strains in indigenous goats remains poorly characterized. This study evaluated six distinct PPRV strains (four lineage-IV, two lineage-III) in experimentally infected Ethiopian goats. Twenty-one Central highland breed goats (12–30 months) were randomly allocated into six experimental groups and one control group, with three goats in each group and experimental animals inoculated intranasally (104.5 TCID50 per ml). Clinical, serological, molecular, and pathogenesis data were collected. Results: Five of six strains produced consistent clinical manifestations beginning at 4 days post-infection (dpi), with seroconversion by 7 dpi and viral antigens detectable in nasal swabs from 3 dpi. No substantial lineage-specific differences in virulence were observed between lineage-III and lineage-IV strains. One lineage-IV isolate (38,920/19) produced significantly milder clinical manifestations (P < 0.05) and no seroconversion, suggesting potential strain-level variation. Gross and histopathological findings were consistent across virulent strains, with nasal swabs proving most reliable for early diagnosis. Conclusions: This first comparative study of Ethiopian PPRV strains establishes similar virulence profiles across lineages in local goats, suggesting that lineage classification alone may not predict clinical outcomes—an important finding for regional disease control strategies. The reduced virulence in one strain suggests factors beyond genomic lineage may influence experimental outcomes, though additional work with standardized protocols is needed to confirm these findings. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 17466148 |
| DOI: | 10.1186/s12917-025-05073-4 |