Antimicrobial resistance and characterization of ESBL-producing Escherichia coli from poultry and cattle in Isfahan, Iran.
Uloženo v:
| Název: | Antimicrobial resistance and characterization of ESBL-producing Escherichia coli from poultry and cattle in Isfahan, Iran. |
|---|---|
| Autoři: | Moradzadeh, Farough1 (AUTHOR), Rahimi, Ebrahim1 (AUTHOR) ebrahimrahimi55@yahoo.com, Shakerian, Amir1 (AUTHOR) |
| Zdroj: | BMC Veterinary Research. 10/2/2025, Vol. 21 Issue 1, p1-7. 7p. |
| Druh dokumentu: | Article |
| Témata: | Multidrug resistance, Beta lactamases, Drug resistance in microorganisms, Cattle, Poultry, Zoonoses |
| Geografický termín: | Isfahan (Iran), Iran |
| Author-Supplied Keywords: | Antimicrobial resistance (AMR) Biological Sciences Genetics Agricultural and Veterinary Sciences Veterinary Sciences Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) Multidrug resistance (MDR) |
| Abstrakt: | Background: The rise of antimicrobial resistance (AMR), particularly from extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli, poses a global public health threat. This study investigated the prevalence, genetic characteristics, and antimicrobial resistance patterns of ESBL-producing E. coli isolated from free-range poultry and clinically healthy cattle in Isfahan, Iran. Method: A total of 300 fecal samples were collected between May 2023 to May 2024, including 150 from smallholder poultry farms and 150 from a mix of dairy and beef cattle farms. Twenty privately owned farms (10 poultry and 10 cattle) were randomly selected using a stratified random sampling approach. All animals were clinically assessed by a licensed veterinarian and found to be healthy at the time of sampling. Samples were screened for ESBL production, and antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method according to CLSI 2018 guidelines. Phenotypic confirmation of ESBLs was conducted using the double-disk synergy test (DDST). Genotypic analysis targeting blaCTX-M, blaTEM, blaAmpC, mcr-1, qnrS, qnrB, and aac(6')-Ib-cr genes and Class 1 and 2 integrons was conducted via PCR. Results: ESBL-producing E. coli was detected in 43.33% of poultry and 36.67% of cattle samples. The most common gene was blaCTX-M (30.77% poultry, 45.45% cattle). Multidrug resistance (MDR) occurred in 53.85% and 54.55% of poultry and cattle isolates, respectively. No statistically significant differences were observed between species (p > 0.05). Conclusion: The findings underscore the potential zoonotic risk of ESBL- and MDR-producing E. coli from livestock. Strengthened antibiotic stewardship and molecular surveillance in Iranian agriculture are essential. [ABSTRACT FROM AUTHOR] |
| Copyright of BMC Veterinary Research is the property of BioMed Central and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Author Affiliations: | 1https://ror.org/02tbw3b35 Department of Veterinary, ShK.C., Islamic Azad University, Shahrekord, Iran |
| Full Text Word Count: | 4932 |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-025-04996-2 |
| Přístupové číslo: | 188451287 |
| Databáze: | Veterinary Source |
|
Nepřihlášeným uživatelům se plný text nezobrazuje
Pro úplný přístup je nutné se přihlásit.
|
|
| Abstrakt: | Background: The rise of antimicrobial resistance (AMR), particularly from extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli, poses a global public health threat. This study investigated the prevalence, genetic characteristics, and antimicrobial resistance patterns of ESBL-producing E. coli isolated from free-range poultry and clinically healthy cattle in Isfahan, Iran. Method: A total of 300 fecal samples were collected between May 2023 to May 2024, including 150 from smallholder poultry farms and 150 from a mix of dairy and beef cattle farms. Twenty privately owned farms (10 poultry and 10 cattle) were randomly selected using a stratified random sampling approach. All animals were clinically assessed by a licensed veterinarian and found to be healthy at the time of sampling. Samples were screened for ESBL production, and antimicrobial susceptibility testing (AST) was performed using the Kirby-Bauer disk diffusion method according to CLSI 2018 guidelines. Phenotypic confirmation of ESBLs was conducted using the double-disk synergy test (DDST). Genotypic analysis targeting blaCTX-M, blaTEM, blaAmpC, mcr-1, qnrS, qnrB, and aac(6')-Ib-cr genes and Class 1 and 2 integrons was conducted via PCR. Results: ESBL-producing E. coli was detected in 43.33% of poultry and 36.67% of cattle samples. The most common gene was blaCTX-M (30.77% poultry, 45.45% cattle). Multidrug resistance (MDR) occurred in 53.85% and 54.55% of poultry and cattle isolates, respectively. No statistically significant differences were observed between species (p > 0.05). Conclusion: The findings underscore the potential zoonotic risk of ESBL- and MDR-producing E. coli from livestock. Strengthened antibiotic stewardship and molecular surveillance in Iranian agriculture are essential. [ABSTRACT FROM AUTHOR] |
|---|---|
| ISSN: | 17466148 |
| DOI: | 10.1186/s12917-025-04996-2 |