APPLICATION OF MICROSATELLITE-BASED PARENTAGE VERIFICATION USING VeriSireTM IN SELECTED CATTLE FARMS IN THE PHILIPPINES.
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| Titel: | APPLICATION OF MICROSATELLITE-BASED PARENTAGE VERIFICATION USING VeriSireTM IN SELECTED CATTLE FARMS IN THE PHILIPPINES. |
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| Autoren: | Garcia, Millen Angeline M.1 gmillenangeline@gmail.com, Dela Cruz, Yujiner C.1, Gajeton, Maureen B.1, Yusi, Maria Rica M.1, Reyes, Melinda N.1, Flores, Ester B.1 |
| Quelle: | Philippine Journal of Veterinary & Animal Sciences. Jul-Dec2024, Vol. 50 Issue 2, p58-69. 12p. |
| Publikationsart: | Article |
| Schlagworte: | Animal pedigrees, Livestock breeding, Animal industry, Capillary electrophoresis, Livestock breeds |
| Author-Supplied Keywords: | cattle microsatellite markers parentage verification pedigree VeriSire |
| Abstract: | Parentage verification using microsatellite markers is yet to be fully appreciated in the local cattle industry. Breeder experiences have proven that parentage testing, in combination with well-run breeding programs, can ensure accurate pedigrees which are necessary for genetic improvement and to avoid unintentional inbreeding. However, keeping such records is a widespread challenge among cattle raisers specifically in keeping their bull records. Thus, this study aims to validate the pedigree of animals in farms with different breeding methods using VeriSireTM - a DNA-based test to verify the true sire of a calf. Blood and hair follicle samples were collected from four local cattle farms. Subsequent processes of DNA extraction, PCR amplification using 16 markers pooled into 4 panels, gel electrophoresis, capillary electrophoresis, and parentage assignment were performed. The study underscores the practicality of using gDNA extracted from hair follicle samples as an alternative to blood samples. Our results revealed that through parentage verification, the pedigree information of the target animals can be corrected using VeriSireTM. The analysis identified the true sire of 20 animals in a farm utilizing more than 20 bulls for breeding. In three other farms that utilize AI and a combination of both, sire mismatches were identified, and the pedigree of 26 animals was corrected and validated. These results are strong indications that either one breeding method can be compromised when breeding programs are not properly implemented. This research emphasizes the importance and efficacy of rigorous pedigree verification methods in ensuring the integrity and genetic progress of breeding programs in the livestock industry. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Animal Breeding and Genomics Section, Philippine Carabao Center - National Headquarters and Genepool, Science City of Muñoz Nueva Ecija. |
| ISSN: | 0115-2173 |
| Dokumentencode: | 183659868 |
| Datenbank: | Veterinary Source |
| Abstract: | Parentage verification using microsatellite markers is yet to be fully appreciated in the local cattle industry. Breeder experiences have proven that parentage testing, in combination with well-run breeding programs, can ensure accurate pedigrees which are necessary for genetic improvement and to avoid unintentional inbreeding. However, keeping such records is a widespread challenge among cattle raisers specifically in keeping their bull records. Thus, this study aims to validate the pedigree of animals in farms with different breeding methods using VeriSireTM - a DNA-based test to verify the true sire of a calf. Blood and hair follicle samples were collected from four local cattle farms. Subsequent processes of DNA extraction, PCR amplification using 16 markers pooled into 4 panels, gel electrophoresis, capillary electrophoresis, and parentage assignment were performed. The study underscores the practicality of using gDNA extracted from hair follicle samples as an alternative to blood samples. Our results revealed that through parentage verification, the pedigree information of the target animals can be corrected using VeriSireTM. The analysis identified the true sire of 20 animals in a farm utilizing more than 20 bulls for breeding. In three other farms that utilize AI and a combination of both, sire mismatches were identified, and the pedigree of 26 animals was corrected and validated. These results are strong indications that either one breeding method can be compromised when breeding programs are not properly implemented. This research emphasizes the importance and efficacy of rigorous pedigree verification methods in ensuring the integrity and genetic progress of breeding programs in the livestock industry. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 01152173 |