First Report of Zoonotic Cryptosporidium parvum Subtype IIaA15G2R1 in Dogs in Türkiye.
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| Názov: | First Report of Zoonotic Cryptosporidium parvum Subtype IIaA15G2R1 in Dogs in Türkiye. |
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| Autori: | Ayan, Adnan1 dradnanayan@gmail.com, Celik, Burcak Aslan2, Celik, Ozgur Yasar3, Akyildiz, Gurkan4, Kilinc, Ozlem Orunc5, Ayan, Ozge Oktay6, Oguz, Fatma Ertas7, Goz, Yasar8, Yuksek, Nazmi9, Yilmaz, Ali Bilgin10, Myrzhiyeva, Assem11, Akhmetzhanova, Aizhan12, Uslu, Ugur13 |
| Zdroj: | Pakistan Veterinary Journal. 2024, Vol. 44 Issue 4, p1263-1268. 6p. |
| Druh dokumentu: | Article |
| Predmet: | Cryptosporidium parvum, Feral dogs, Public spaces, Cryptosporidium, Sequence analysis |
| Author-Supplied Keywords: | Molecular characterization van Türkiye Subtype iiaa15g2r1 Dog |
| Abstrakt: | Cryptosporidium (C.) is an opportunistic protozoan causing gastrointestinal illness in both humans and animals, leading to acute or chronic diarrhea and even death. The study aimed to investigate the prevalence and subtyping of Cryptosporidium spp. in shelter dogs in Van province, Türkiye. For microscopic identification of this parasite, a total of 300 fecal samples were collected and stained with Kinyoun's acid-fast method. For molecular analysis, the positive samples were subjected to DNA extraction and SSU rRNA gene of Cryptosporidium spp. was amplified using nested PCR. The microscopic examination revealed a 4.67% prevalence of Cryptosporidium spp. Sequence analysis indicated all samples were positive to C. parvum. In addition, GP60 gene was also amplified and C. parvum subtypes IIaA15G2R1 was confirmed by analyzing the obtained sequences. All the sequences of SSU rRNA and GP60 were deposited in GenBank. To our knowledge, Cryptosporidium parvum subtypes IIaA15G2R1 have been reported first time in dogs in Türkiye. It is recommended to implement control strategies by awareness campaign, preventing stray dogs from freely entering public areas, and proper disposal of dog feces. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Department of Genetics, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Türkiye 2Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye 3Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye 4Department of Basic Health Sciences, Health Sciences Faculty, Marmara University, Istanbul, Türkiye 5Ozalp Vocational School, Van Yuzuncu Yil University, Van, Türkiye 6Department of Parasitology, Faculty of Medicine, Van Yuzuncu Yil University, Van, Türkiye 7Tuzluca Vocational School, Igdir University, Igdir, Türkiye 8Department of Nutrition and Dietetics, Faculty of Health Sciences, Van Yuzuncu Yil University, Van, Türkiye 9Department of Internal Medicine, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Türkiye 10Faculty of Health, Van Yuzuncu Yil University, Van, Türkiye 11Veterinary Sciences, Kazakh National Agrarian Research University, Kazakhstan 12Department of Veterinary, Faculty of Agrarian, Shakarim State University of Semey, Kazakhstan 13Department of Parasitology, Faculty of Medicine, University of Istanbul Medeniyet, Istanbul, Türkiye |
| ISSN: | 0253-8318 |
| DOI: | 10.29261/pakvetj/2024.274 |
| Prístupové číslo: | 182205090 |
| Databáza: | Veterinary Source |
| Abstrakt: | Cryptosporidium (C.) is an opportunistic protozoan causing gastrointestinal illness in both humans and animals, leading to acute or chronic diarrhea and even death. The study aimed to investigate the prevalence and subtyping of Cryptosporidium spp. in shelter dogs in Van province, Türkiye. For microscopic identification of this parasite, a total of 300 fecal samples were collected and stained with Kinyoun's acid-fast method. For molecular analysis, the positive samples were subjected to DNA extraction and SSU rRNA gene of Cryptosporidium spp. was amplified using nested PCR. The microscopic examination revealed a 4.67% prevalence of Cryptosporidium spp. Sequence analysis indicated all samples were positive to C. parvum. In addition, GP60 gene was also amplified and C. parvum subtypes IIaA15G2R1 was confirmed by analyzing the obtained sequences. All the sequences of SSU rRNA and GP60 were deposited in GenBank. To our knowledge, Cryptosporidium parvum subtypes IIaA15G2R1 have been reported first time in dogs in Türkiye. It is recommended to implement control strategies by awareness campaign, preventing stray dogs from freely entering public areas, and proper disposal of dog feces. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 02538318 |
| DOI: | 10.29261/pakvetj/2024.274 |