Molecular characterization of VP1 gene during the foot and mouth disease virus outbreak in East Java, Indonesia, in 2022.
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| Název: | Molecular characterization of VP1 gene during the foot and mouth disease virus outbreak in East Java, Indonesia, in 2022. |
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| Autoři: | Dinana, Zayyin1,2 dzayyin@gmail.com, Suwarno, Suwarno3 suwarno@fkh.unair.ac.id, Mustofa, Imam4 imam.mustofa@fkh.unair.ac.id, Rahmahani, Jola3 jola_rahmahani@yahoo.co.id, Kusnoto, Kusnoto5 kusnoto@fkh.unair.ac.id, Maharani, Aussie Tahta2 aussietahta30@gmail.com, Fitria, Anisa Lailatul2 anisalailatulfitria@gmail.com, Witaningrum, Adiana Mutamsari6 adianamutam@gmail.com, Maulana, Firdausy Kurnia1,7 firdausykurnia@gmail.com, Said, Nur Saidah1,8 nursaidah@unsulbar.ac.id, Fahrodi, Deka Uli1,8 dekaulifahrodi@unsulbar.ac.id, Rantam, Fedik Abdul3,7,9 fedik-a-r@fkh.unair.ac.id |
| Zdroj: | Veterinary World. Nov2024, Vol. 17 Issue 11, p2469-2476. 8p. |
| Druh dokumentu: | Article |
| Témata: | Foot & mouth disease virus, Foot & mouth disease, Whole genome sequencing, Viral proteins, Beef cattle |
| Geografický termín: | Indonesia |
| Author-Supplied Keywords: | cattle foot and mouth disease virus phylogenetic tree viral proteins 1 |
| Abstrakt: | Background and Aim: Foot and mouth disease (FMD) is highly contagious in cloven-hoofed animals, and it causes outbreaks in Indonesia and several countries worldwide. This disease is caused by the FMD virus (FMDV), which belongs to the genus Aphthovirus and family Picornaviridae. In 1990, the World Organization for Animal Health Office International des Epizooties recognized Indonesia as an FMD-free country. A new FMDV outbreak in Indonesia was reported in April 2022 and confirmed in May 2022, resulting in economic losses to the beef cattle sector. This study aimed to determine the genotype and amino acid content of viral proteins (VP1) gene. Materials and Methods: Samples were obtained from vesicle swabs from the mouth and feet of cattle in Banyuwangi Regency, Lamongan Regency, and Surabaya City, East Java, Indonesia. Samples were identified using one-step reverse transcriptase-polymerase chain reaction with a pair of specific primers encoding the VP1O serotype with a target of 1165 bp. Results: Sequencing revealed that the FMDV subtype belonged to the O/ME-SA/Ind2001e. Phylogenetic analysis showed that our isolate was 100% amino acid-identical to the Indonesian outbreak isolates from 2022 and 95% identical to isolates from Southeast Asia. The amino acid substitutions found in the G-H Loop of the VP1were S134C, D138E, T140A, and A156T. Only the K135Q mutation was detected in Lamongan. Conclusion: The spread of the subtype O/ME-SA/Ind2001e in South-east Asia caused an outbreak in Indonesia due to less stringent animal traffic control measures. Surveillance studies and whole-genome sequence analyses are important for monitoring FMDV genetics in Indonesia. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Doctoral Program of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 2Department of Viral Diarrhea, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia. 3Division of Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 4Division of Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 5Division of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 6Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia. 7Airlangga Disease Prevention and Research Center, Universitas Airlangga, Surabaya, Indonesia. 8Department of Animal Husbandry, Faculty of Animal Husbandry and Fisheries, Universitas Sulawesi Barat, Majene, Indonesia. 9Research Center for Vaccine Technology and Development, Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia. |
| ISSN: | 0972-8988 |
| DOI: | 10.14202/vetworld.2024.2469-2476 |
| Přístupové číslo: | 181265648 |
| Databáze: | Veterinary Source |
| Abstrakt: | Background and Aim: Foot and mouth disease (FMD) is highly contagious in cloven-hoofed animals, and it causes outbreaks in Indonesia and several countries worldwide. This disease is caused by the FMD virus (FMDV), which belongs to the genus Aphthovirus and family Picornaviridae. In 1990, the World Organization for Animal Health Office International des Epizooties recognized Indonesia as an FMD-free country. A new FMDV outbreak in Indonesia was reported in April 2022 and confirmed in May 2022, resulting in economic losses to the beef cattle sector. This study aimed to determine the genotype and amino acid content of viral proteins (VP1) gene. Materials and Methods: Samples were obtained from vesicle swabs from the mouth and feet of cattle in Banyuwangi Regency, Lamongan Regency, and Surabaya City, East Java, Indonesia. Samples were identified using one-step reverse transcriptase-polymerase chain reaction with a pair of specific primers encoding the VP1O serotype with a target of 1165 bp. Results: Sequencing revealed that the FMDV subtype belonged to the O/ME-SA/Ind2001e. Phylogenetic analysis showed that our isolate was 100% amino acid-identical to the Indonesian outbreak isolates from 2022 and 95% identical to isolates from Southeast Asia. The amino acid substitutions found in the G-H Loop of the VP1were S134C, D138E, T140A, and A156T. Only the K135Q mutation was detected in Lamongan. Conclusion: The spread of the subtype O/ME-SA/Ind2001e in South-east Asia caused an outbreak in Indonesia due to less stringent animal traffic control measures. Surveillance studies and whole-genome sequence analyses are important for monitoring FMDV genetics in Indonesia. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 09728988 |
| DOI: | 10.14202/vetworld.2024.2469-2476 |