Enhancing impact of dietary nano formulated quercetin on laying performance: egg quality, oxidative stability of stored eggs, intestinal immune and antioxidants related genes expression.
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| Title: | Enhancing impact of dietary nano formulated quercetin on laying performance: egg quality, oxidative stability of stored eggs, intestinal immune and antioxidants related genes expression. |
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| Authors: | Al-Khalaifah, Hanan S.1 (AUTHOR), Ibrahim, Doaa2 (AUTHOR), Kamel, Asmaa EL-Sayed2 (AUTHOR), Al-Nasser, Afaf1 (AUTHOR), Abdelwarith, Abdelwahab A.3 (AUTHOR), Roushdy, Elshimaa M.4 (AUTHOR), Sheraiba, Nagwa I.5 (AUTHOR), Shafik, Basant M.6 (AUTHOR), El-Badry, Sara M.7 (AUTHOR), Younis, Elsayed M.3 (AUTHOR), Mamdouh, Maha8 (AUTHOR), Yassin, Engy Mohamed Mohamed9 (AUTHOR), Davies, Simon J.10 (AUTHOR), Kishawy, Asmaa T. Y.2 (AUTHOR) no.dispair2000@gmail.com |
| Source: | BMC Veterinary Research. 10/29/2024, Vol. 20 Issue 1, p1-19. 19p. |
| Document Type: | Article |
| Author-Supplied Keywords: | Antioxidant Egg quality Laying performance Lipid peroxidation Quercetin nano formulated Storage |
| Abstract: | Background: Nutritional interventions with natural antioxidants can provide a pragmatic solution for modifying hens’ performance and maintaining oxidative stability of eggs during storage. Quercetin is the most abundant flavonoids with potent antioxidant and immune stimulant activities. The concept of incorporating of quercetin, as potent antioxidant and immunostimulant, into effective nano-carriers (QNPs) has promoted their bioavailability and stability thus, their effectiveness for the first time were assessed on laying hens’ performance and immunity, eggs quality during storage. Four hundred 12-weeks-old Hy-line brown laying hens were distributed to four experimental groups: control group fed basal diets, and other 3 groups fed basal diets fortified with 100, 200 and 300 mg/kg QNPs for 60 weeks. Results: Laying performance and quality of laid eggs were improved as expressed by elevated laying rate, egg mass %, eggs weight and yolk weight in QNPs200 and 300. Fortification of QNPs300 remarkably decreased layers serum total cholesterol concurrently with decreased egg yolk saturated fatty acids and cholesterol while increased polyunsaturated fatty acids. Over- 45 days storage period, QNPs enhanced phospholipids, total phenolics and flavonoids, total antioxidant activity (T-AOC) simultaneous with decreased MDA content in eggs. Furthermore, enhanced immune response was detected in both in serum and intestine of QNPs fed hens as reflected by higher lysozymes activity, IgM, IgG and phagocytic index and demotion of NO together with AvBD 6–12, IL-10, IgM and ATg 5-7-12 upregulation and downregulation of IL-1β and TNF-α especially at QNPs200 and 300. Intestinal redox balance was modified via decreasing H2O2 and MDA simultaneous with upregulation of catalase, SOD, GSH-Px, HO-1 and NQO1 in groups fed higher doses of QNPs. Conclusions: QNPs supplementation provides a new nutritional strategy towards increasing hen performance, fortification of eggs with natural antioxidants that prevents egg quality deterioration during storage. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1https://ror.org/041tgg678 Environment and Life Sciences Research Center, Kuwait Institute for Scientific Research, P.O. Box:24885, 13109, Safat, Kuwait 2https://ror.org/053g6we49 Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig city, Egypt 3https://ror.org/02f81g417 Department of Zoology, College of Science, King Saudi University, 11451, Riyadh, Saudi Arabia 4https://ror.org/053g6we49 Department of Animal Wealth Development, Animal Breeding, and Production, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig city, Egypt 5https://ror.org/05p2q6194 Department of Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, University of Sadat City, 32897, Sadat city, Egypt 6https://ror.org/03tn5ee41 Department of Animal Wealth Development, Animal and Poultry Production, Faculty of Veterinary Medicine, Benha University, P.O. Box 13736, Toukh city, Qalyubia, Egypt 7https://ror.org/053g6we49 Department of Animal Wealth Development, Veterinary Genetics & Genetic Engineering, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig city, Egypt 8https://ror.org/03tn5ee41 Department of Physiology, Faculty of Veterinary Medicine, Benha University, P.O. Box 13736, Toukh city, Qalyubia, Egypt 9https://ror.org/053g6we49 Department of Biochemistry and molecular biology, Faculty of Veterinary Medicine, Zagazig University, 44511, Zagazig city, Egypt 10https://ror.org/03bea9k73 Aquaculture Nutrition Research Unit (ANRU), Carna Research Station, College of Science and Engineering, Ryan Institute, University of Galway, Galway, Ireland |
| Full Text Word Count: | 14846 |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-024-04327-x |
| Accession Number: | 180667145 |
| Database: | Veterinary Source |
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| Abstract: | Background: Nutritional interventions with natural antioxidants can provide a pragmatic solution for modifying hens’ performance and maintaining oxidative stability of eggs during storage. Quercetin is the most abundant flavonoids with potent antioxidant and immune stimulant activities. The concept of incorporating of quercetin, as potent antioxidant and immunostimulant, into effective nano-carriers (QNPs) has promoted their bioavailability and stability thus, their effectiveness for the first time were assessed on laying hens’ performance and immunity, eggs quality during storage. Four hundred 12-weeks-old Hy-line brown laying hens were distributed to four experimental groups: control group fed basal diets, and other 3 groups fed basal diets fortified with 100, 200 and 300 mg/kg QNPs for 60 weeks. Results: Laying performance and quality of laid eggs were improved as expressed by elevated laying rate, egg mass %, eggs weight and yolk weight in QNPs200 and 300. Fortification of QNPs300 remarkably decreased layers serum total cholesterol concurrently with decreased egg yolk saturated fatty acids and cholesterol while increased polyunsaturated fatty acids. Over- 45 days storage period, QNPs enhanced phospholipids, total phenolics and flavonoids, total antioxidant activity (T-AOC) simultaneous with decreased MDA content in eggs. Furthermore, enhanced immune response was detected in both in serum and intestine of QNPs fed hens as reflected by higher lysozymes activity, IgM, IgG and phagocytic index and demotion of NO together with AvBD 6–12, IL-10, IgM and ATg 5-7-12 upregulation and downregulation of IL-1β and TNF-α especially at QNPs200 and 300. Intestinal redox balance was modified via decreasing H2O2 and MDA simultaneous with upregulation of catalase, SOD, GSH-Px, HO-1 and NQO1 in groups fed higher doses of QNPs. Conclusions: QNPs supplementation provides a new nutritional strategy towards increasing hen performance, fortification of eggs with natural antioxidants that prevents egg quality deterioration during storage. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 17466148 |
| DOI: | 10.1186/s12917-024-04327-x |