Pseudomonas putida infection induces immune-antioxidant, hepato-renal, ethological, and histopathological/immunohistochemical disruptions in Oreochromis niloticus: the palliative role of titanium dioxide nanogel.
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| Title: | Pseudomonas putida infection induces immune-antioxidant, hepato-renal, ethological, and histopathological/immunohistochemical disruptions in Oreochromis niloticus: the palliative role of titanium dioxide nanogel. |
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| Authors: | Rahman, Afaf N. Abdel1 (AUTHOR) afne56@gmail.com, Mahboub, Heba H.1 (AUTHOR) hhhmb@yahoo.com, Elshopakey, Gehad E.2 (AUTHOR), Darwish, Mahmoud I. M.3 (AUTHOR), Gharib, Heba Said Abdel-Rahman4 (AUTHOR), Shaalan, Mohamed5,6 (AUTHOR) mohamedibrahim@cu.edu.eg, Fahmy, Esraa M.7 (AUTHOR), Abdel-Ghany, Heba M.8 (AUTHOR), Ismail, Sameh H.9 (AUTHOR), Elsheshtawy, Hassnaa Mahmoud10 (AUTHOR) |
| Source: | BMC Veterinary Research. 4/1/2024, Vol. 20 Issue 1, p1-13. 13p. |
| Document Type: | Article |
| Subjects: | Antioxidants, Pseudomonas putida, Pseudomonas diseases, Nile tilapia, Titanium dioxide, Oxidant status, Pathogenic bacteria |
| Author-Supplied Keywords: | Bacterial infection Health status Immune response Titanium dioxide nanogel |
| Abstract: | Background: Pseudomonas putida is a pathogenic bacterium that induces great losses in fishes, including Nile tilapia (Oreochromis niloticus). Currently, the application of nanomaterials in aquaculture practices has gained more success as it endows promising results in therapies compared to traditional protocols. Objective: Therefore, the current perspective is considered the first report to assess the anti-bacterial efficacy of titanium dioxide nanogel (TDNG) against Pseudomonas putida (P. putida) in Nile tilapia. Methods: The fish (n = 200; average body weight: 47.50±1.32 g) were allocated into four random groups (control, TDNG, P. putida, and TDNG + P. putida), where 0.9 mg/L of TDNG was applied as bath treatment for ten days. Results: Outcomes revealed that P. putida infection caused ethological alterations (surfacing, abnormal movement, and aggression) and depression of immune-antioxidant variables (complement 3, lysozyme activity, total antioxidant capacity, superoxide dismutase, and reduced glutathione content). Additionally, a substantial elevation in hepatorenal biomarkers (aspartate and alanine aminotransferases and creatinine) with clear histopathological changes and immuno-histochemical alterations (very weak BCL-2 and potent caspase-3 immuno-expressions) were seen. Surprisingly, treating P. putida-infected fish with TDNG improved these variables and obvious restoration of the tissue architectures. Conclusion: Overall, this report encompasses the key role of TDNG as an anti-bacterial agent for controlling P. putida infection and improving the health status of Nile tilapia. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1https://ror.org/053g6we49 Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt 2https://ror.org/01k8vtd75 Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, PO Box 35516, Mansoura, Dakahlia, Egypt 3https://ror.org/053g6we49 Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt 4https://ror.org/053g6we49 Department of Behaviour and Management of Animal, Poultry, and Aquatics, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt 5https://ror.org/03q21mh05 Department of Pathology, Faculty of Veterinary Medicine, Cairo University, PO Box 12211, Giza, Egypt 6Polymer Institute, Slovak academy of sciences, Dúbravská cesta 9, 845 41, Bratislava, Slovakia 7https://ror.org/053g6we49 Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt 8https://ror.org/053g6we49 Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt 9https://ror.org/03q21mh05 Faculty of Nanotechnology for Postgraduate Studies, Cairo University, PO Box 12588, Sheikh Zayed Branch Campus, Sheikh Zayed City, Giza, Egypt 10https://ror.org/02m82p074 Department of Fish Diseases and Management, Faculty of Veterinary Medicine, Suez Canal University, PO Box 41522, Ismailia, Egypt |
| Full Text Word Count: | 7101 |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-024-03972-6 |
| Accession Number: | 176353919 |
| Database: | Veterinary Source |
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| Abstract: | Background: Pseudomonas putida is a pathogenic bacterium that induces great losses in fishes, including Nile tilapia (Oreochromis niloticus). Currently, the application of nanomaterials in aquaculture practices has gained more success as it endows promising results in therapies compared to traditional protocols. Objective: Therefore, the current perspective is considered the first report to assess the anti-bacterial efficacy of titanium dioxide nanogel (TDNG) against Pseudomonas putida (P. putida) in Nile tilapia. Methods: The fish (n = 200; average body weight: 47.50±1.32 g) were allocated into four random groups (control, TDNG, P. putida, and TDNG + P. putida), where 0.9 mg/L of TDNG was applied as bath treatment for ten days. Results: Outcomes revealed that P. putida infection caused ethological alterations (surfacing, abnormal movement, and aggression) and depression of immune-antioxidant variables (complement 3, lysozyme activity, total antioxidant capacity, superoxide dismutase, and reduced glutathione content). Additionally, a substantial elevation in hepatorenal biomarkers (aspartate and alanine aminotransferases and creatinine) with clear histopathological changes and immuno-histochemical alterations (very weak BCL-2 and potent caspase-3 immuno-expressions) were seen. Surprisingly, treating P. putida-infected fish with TDNG improved these variables and obvious restoration of the tissue architectures. Conclusion: Overall, this report encompasses the key role of TDNG as an anti-bacterial agent for controlling P. putida infection and improving the health status of Nile tilapia. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 17466148 |
| DOI: | 10.1186/s12917-024-03972-6 |