A Profile of Single Nucleotide Polymorphisms in Fecundity Genes Among Iranian Sheep Breeds by Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Method.
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| Název: | A Profile of Single Nucleotide Polymorphisms in Fecundity Genes Among Iranian Sheep Breeds by Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Method. |
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| Autoři: | Potki, P.1 p.potki@abrii.ac.ir, Mirhoseini, S. Z.2, Afraz, F.3, Vahidi, S. M. F.3 |
| Zdroj: | Iranian Journal of Applied Animal Science. Jun2020, Vol. 10 Issue 2, p265-285. 21p. |
| Druh dokumentu: | Article |
| Témata: | Sheep breeds, Group of Seven (Organization), Single nucleotide polymorphisms, Bone morphogenetic proteins, Sheep breeding, Genetic polymorphisms, Polymerase chain reaction, Ovulation |
| Geografický termín: | Group of Eight countries |
| Author-Supplied Keywords: | BMP15 BMPR1B GDF9 PCR-RFLP sheep SNP |
| Abstrakt: | Ovulation rate and litter size are traits of fecundity and have a very remarkable economic value that leads to an increase in the production of meat, wool and milk. Therefore, exploring on the candidate and functional genes for these traits is very important. Growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15) and bone morphogenetic protein receptor-1B (BMPR1B) are the most important of these genes containing single nucleotide polymorphisms (SNPs) markers that are associated with high fertility in sheeps. The aim of this study was to determine the allelic and genotypic frequencies of seven of these SNPs including; G1, G4, G7 and G8 in GDF9 gene, B2 and B4 in BMP15 gene and FecB in BMPR1B gene among 532 sheeps from eleven of Iranian native breeds using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. For each mutation region, polymerase chain reactions and digestion reactions with specific endonuclease enzymes, were performed. The results showed that G1 mutation presents among ten breeds except Zandi, G4 mutation in all breeds, and the FecB mutation in two breeds; Kalakuei and Zandi. The G7, G8, B2 and B4 mutations were not observed in any of breeds, and all sheeps were homozygote wild type for these four positions. [ABSTRACT FROM AUTHOR] |
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| Author Affiliations: | 1Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran 2Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran 3Department of Animal Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran |
| ISSN: | 2251-628X |
| Přístupové číslo: | 143844833 |
| Databáze: | Veterinary Source |
| Abstrakt: | Ovulation rate and litter size are traits of fecundity and have a very remarkable economic value that leads to an increase in the production of meat, wool and milk. Therefore, exploring on the candidate and functional genes for these traits is very important. Growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15) and bone morphogenetic protein receptor-1B (BMPR1B) are the most important of these genes containing single nucleotide polymorphisms (SNPs) markers that are associated with high fertility in sheeps. The aim of this study was to determine the allelic and genotypic frequencies of seven of these SNPs including; G1, G4, G7 and G8 in GDF9 gene, B2 and B4 in BMP15 gene and FecB in BMPR1B gene among 532 sheeps from eleven of Iranian native breeds using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. For each mutation region, polymerase chain reactions and digestion reactions with specific endonuclease enzymes, were performed. The results showed that G1 mutation presents among ten breeds except Zandi, G4 mutation in all breeds, and the FecB mutation in two breeds; Kalakuei and Zandi. The G7, G8, B2 and B4 mutations were not observed in any of breeds, and all sheeps were homozygote wild type for these four positions. [ABSTRACT FROM AUTHOR] |
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| ISSN: | 2251628X |