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Exploring Protein Structure and Dynamics through a Project-Oriented Biochemistry Laboratory Module

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Bibliographic Details
Title: Exploring Protein Structure and Dynamics through a Project-Oriented Biochemistry Laboratory Module
Language: English
Authors: Lipchock, James M., Ginther, Patrick S., Douglas, Bonnie B., Bird, Kelly E., Loria, J. Patrick
Source: Biochemistry and Molecular Biology Education. Sep-Oct 2017 45(5):403-410.
Availability: Wiley-Blackwell. 350 Main Street, Malden, MA 02148. Tel: 800-835-6770; Tel: 781-388-8598; Fax: 781-388-8232; e-mail: cs-journals@wiley.com; Web site: http://www.wiley.com/WileyCDA
Peer Reviewed: Y
Page Count: 8
Publication Date: 2017
Sponsoring Agency: National Science Foundation (NSF)
Contract Number: MCB1121372
Document Type: Journal Articles
Reports - Descriptive
Education Level: Higher Education
Descriptors: Biochemistry, Undergraduate Students, Student Research, Genetics, Kinetics, Laboratory Experiments, Molecular Structure, Active Learning, Student Projects
DOI: 10.1002/bmb.21056
ISSN: 1470-8175
Abstract: Here, we present a 10-week project-oriented laboratory module designed to provide a course-based undergraduate research experience in biochemistry that emphasizes the importance of biomolecular structure and dynamics in enzyme function. This module explores the impact of mutagenesis on an important active site loop for a biomedically-relevant human enzyme, protein tyrosine phosphatase 1B (PTP1B). Over the course of the semester students guide their own mutant of PTP1B from conception to characterization in a cost-effective manner and gain exposure to fundamental techniques in biochemistry, including site-directed DNA mutagenesis, bacterial recombinant protein expression, affinity column purification, protein quantitation, SDS-PAGE, and enzyme kinetics. This project-based approach allows an instructor to simulate a research setting and prepare students for productive research beyond the classroom. Potential modifications to expand or contract this module are also provided.
Abstractor: As Provided
Entry Date: 2017
Accession Number: EJ1154857
Database: ERIC
Description
Abstract:Here, we present a 10-week project-oriented laboratory module designed to provide a course-based undergraduate research experience in biochemistry that emphasizes the importance of biomolecular structure and dynamics in enzyme function. This module explores the impact of mutagenesis on an important active site loop for a biomedically-relevant human enzyme, protein tyrosine phosphatase 1B (PTP1B). Over the course of the semester students guide their own mutant of PTP1B from conception to characterization in a cost-effective manner and gain exposure to fundamental techniques in biochemistry, including site-directed DNA mutagenesis, bacterial recombinant protein expression, affinity column purification, protein quantitation, SDS-PAGE, and enzyme kinetics. This project-based approach allows an instructor to simulate a research setting and prepare students for productive research beyond the classroom. Potential modifications to expand or contract this module are also provided.
ISSN:1470-8175
DOI:10.1002/bmb.21056