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Optimització del diagnòstic microbiològic de la bacterièmia en un hospital sense atenció continuada. Aplicació d’una PCR Multiplex sobre frasc d’hemocultiu

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Název: Optimització del diagnòstic microbiològic de la bacterièmia en un hospital sense atenció continuada. Aplicació d’una PCR Multiplex sobre frasc d’hemocultiu
Autoři: Gil Fortuño, María
Přispěvatelé: University/Department: Universitat Jaume I. Escola de Doctorat
Thesis Advisors: Moreno Muñoz, Rosario, Maciá Soler, Maria Loreto
Zdroj: TDX (Tesis Doctorals en Xarxa)
Informace o vydavateli: Universitat Jaume I, 2022.
Rok vydání: 2022
Fyzický popis: 131 p.
Témata: Diagnòstic, Bacterièmia, Sèpsia, Molecular, Microbiologia, Atenció continuada, Diagnosis, Bacteraemia, Sepsis, Microbiology, Salut i serveis socials
Popis: The microbiological diagnosis of bacteremia is essential to an early establishment of the approppriate treatment. We present a study of 133 patients performing multiplex PCR (FA-BCID) in parallel with conventional blood culture. The diagnosis was advanced by about two days on positive blood culture. The concordance was 79%, 95% CI (66.86), due to the detection of S.pneumoniae which was not isolated. In 22% of patients, the ATB was modified when the PCR result was known. The percentage of polymorphonuclear cells and the age are the only relevant parameters in order to condition a positive PCR result. Experimental PCR was performed in non-positivized blood cultures. In six cases the identification could be considerably advanced. The concordance when PCR was negative with the culture was 82.3%. Especially in hospitals without continuous care, FA-BCID is a good tool that can speed up the diagnosis but cannot replace traditional blood culture.
Description (Translated): Programa de Doctorat en Ciències de la Infermeria
Druh dokumentu: Dissertation/Thesis
Popis souboru: application/pdf
Jazyk: Catalan; Valencian
DOI: 10.6035/14103.2022.252009
Přístupová URL adresa: http://hdl.handle.net/10803/674702
Rights: L'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc/4.0/
Přístupové číslo: edstdx.10803.674702
Databáze: TDX
Popis
Abstrakt:The microbiological diagnosis of bacteremia is essential to an early establishment of the approppriate treatment. We present a study of 133 patients performing multiplex PCR (FA-BCID) in parallel with conventional blood culture. The diagnosis was advanced by about two days on positive blood culture. The concordance was 79%, 95% CI (66.86), due to the detection of S.pneumoniae which was not isolated. In 22% of patients, the ATB was modified when the PCR result was known. The percentage of polymorphonuclear cells and the age are the only relevant parameters in order to condition a positive PCR result. Experimental PCR was performed in non-positivized blood cultures. In six cases the identification could be considerably advanced. The concordance when PCR was negative with the culture was 82.3%. Especially in hospitals without continuous care, FA-BCID is a good tool that can speed up the diagnosis but cannot replace traditional blood culture.
DOI:10.6035/14103.2022.252009