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Understanding the impact of steam pretreatment severity on cellulose ultrastructure, recalcitrance, and hydrolyzability of Norway spruce

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Název: Understanding the impact of steam pretreatment severity on cellulose ultrastructure, recalcitrance, and hydrolyzability of Norway spruce
Autoři: Caputo, Fabio, Al-Rudainy, Basel, Naidjonoka, Polina, Wallberg, Ola, Olsson, Lisbeth, Novy, Vera
Přispěvatelé: Lund University, Faculty of Engineering, LTH, Departments at LTH, Department of Process and Life Science Engineering, Division of Chemical Engineering, Lunds universitet, Lunds Tekniska Högskola, Institutioner vid LTH, Institutionen för processteknik och tillämpad biovetenskap, Avdelningen för kemiteknik, Originator, Lund University, Faculty of Engineering, LTH, LTH Profile areas, LTH Profile Area: The Energy Transition, Lunds universitet, Lunds Tekniska Högskola, LTH profilområden, LTH profilområde: Energiomställningen, Originator
Zdroj: Biomass Conversion and Biorefinery. 14(21):27211-27223
Témata: Engineering and Technology, Materials Engineering, Paper, Pulp and Fiber Technology, Teknik, Materialteknik, Pappers, massa- och fiberteknik
Popis: The efficient use of softwood in biorefineries requires harsh pretreatment conditions to overcome biomass recalcitrance. Following harsh pretreatments, the hemicellulose is solubilized. Here, we studied the material characteristics of Norway spruce following steam pretreatment at six different severities, relating chemical and structural information to the enzymatic hydrolyzability. Steam pretreatment conditions were defined by two different temperatures (180 °C and 210 °C), with and without the addition of various acids (CH3COOH, H3PO4, H2SO4, SO2). Structural knowledge of the streams is a cornerstone for developing an efficient saccharification process. This study combines advanced structural characterizations to gain fundamental understanding of the influence of severity of pretreatment on spruce. Structural knowledge is a cornerstone in developing an effective saccharification process by modulating pretreatment conditions and enzymes employed. Overall structural properties were assessed byscanning electron microscopy. The effect of stream pretreatment severity on lignin and lignin-carbohydrate bonds was investigated by two-dimensional heteronuclear single quantum correlation nuclear magnetic resonance. Finally, cellulose ultrastructure was studied by applying small/wide-angle X-ray scattering. The structural characteristics of the six pretreated softwood substrates were related to the enzymatic hydrolyzability. With increasing pretreatment severity, surface defibrillation, and lignin depolymeryzation were observed. Further, lignin-carbohydrate complexes signals were detected. Cellulose analysis revealed the rearrangement of microfibrils leading to the formation of larger microfibril aggregates. This microfibril rearrangement likely contributed to the observed increase in enzymatic hydrolysis yields as better enzyme accessibility resulted.
Přístupová URL adresa: https://doi.org/10.1007/s13399-022-03405-0
Databáze: SwePub
Popis
Abstrakt:The efficient use of softwood in biorefineries requires harsh pretreatment conditions to overcome biomass recalcitrance. Following harsh pretreatments, the hemicellulose is solubilized. Here, we studied the material characteristics of Norway spruce following steam pretreatment at six different severities, relating chemical and structural information to the enzymatic hydrolyzability. Steam pretreatment conditions were defined by two different temperatures (180 °C and 210 °C), with and without the addition of various acids (CH3COOH, H3PO4, H2SO4, SO2). Structural knowledge of the streams is a cornerstone for developing an efficient saccharification process. This study combines advanced structural characterizations to gain fundamental understanding of the influence of severity of pretreatment on spruce. Structural knowledge is a cornerstone in developing an effective saccharification process by modulating pretreatment conditions and enzymes employed. Overall structural properties were assessed byscanning electron microscopy. The effect of stream pretreatment severity on lignin and lignin-carbohydrate bonds was investigated by two-dimensional heteronuclear single quantum correlation nuclear magnetic resonance. Finally, cellulose ultrastructure was studied by applying small/wide-angle X-ray scattering. The structural characteristics of the six pretreated softwood substrates were related to the enzymatic hydrolyzability. With increasing pretreatment severity, surface defibrillation, and lignin depolymeryzation were observed. Further, lignin-carbohydrate complexes signals were detected. Cellulose analysis revealed the rearrangement of microfibrils leading to the formation of larger microfibril aggregates. This microfibril rearrangement likely contributed to the observed increase in enzymatic hydrolysis yields as better enzyme accessibility resulted.
ISSN:21906823
DOI:10.1007/s13399-022-03405-0