| Přispěvatelé: |
Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Occupational and Environmental Medicine, Lund University, Genetic Occupational and Environmental Medicine, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för arbets- och miljömedicin, Genetisk arbets- och miljömedicin, Originator, Lund University, Faculty of Engineering, LTH, Other operations, LTH, Metalund, Lunds universitet, Lunds Tekniska Högskola, Annan verksamhet, LTH, Metalund, Originator, Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Occupational and Environmental Medicine, Lund University, Applied Mass Spectrometry in Environmental Medicine, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för arbets- och miljömedicin, Tillämpad masspektrometri inom miljömedicin, Originator, Lund University, Faculty of Medicine, Department of Clinical Sciences, Malmö, Occupational and Environmental Dermatology, Lunds universitet, Medicinska fakulteten, Institutionen för kliniska vetenskaper, Malmö, Yrkes- och miljödermatologi, Originator, Lund University, Faculty of Engineering, LTH, Other operations, LTH, Centre for Healthy Indoor Environments, Lunds universitet, Lunds Tekniska Högskola, Annan verksamhet, LTH, Centrum för hälsosamma inomhusmiljöer, CHIE, Originator, Lund University, Faculty of Engineering, LTH, LTH Profile areas, LTH Profile Area: Aerosols, Lunds universitet, Lunds Tekniska Högskola, LTH profilområden, LTH profilområde: Aerosoler, Originator, Lund University, Faculty of Medicine, Department of Laboratory Medicine, Division of Occupational and Environmental Medicine, Lund University, Environmental Epidemiology, Lunds universitet, Medicinska fakulteten, Institutionen för laboratoriemedicin, Avdelningen för arbets- och miljömedicin, Miljöepidemiologi, Originator, Lund University, Profile areas and other strong research environments, Strategic research areas (SRA), EpiHealth: Epidemiology for Health, Lunds universitet, Profilområden och andra starka forskningsmiljöer, Strategiska forskningsområden (SFO), EpiHealth: Epidemiology for Health, Originator, Lund University, Profile areas and other strong research environments, Other Strong Research Environments, LUCC: Lund University Cancer Centre, Lunds universitet, Profilområden och andra starka forskningsmiljöer, Övriga starka forskningsmiljöer, LUCC: Lunds universitets cancercentrum, Originator |
| Popis: |
BACKGROUND: Hexavalent chromium (Cr(Ⅵ)) is classified as a group 1 human carcinogen and increases the risk of lung cancer. Non-coding RNAs (ncRNAs) have key regulatory roles in lung cancer, but less is known about their relation to Cr(Ⅵ) exposure. OBJECTIVES: We aimed to 1) measure the expression of lung cancer-related circulating ncRNAs in exposed workers and controls; 2) assess associations between ncRNAs expression and Cr concentrations in red blood cells (RBC) and urine; and 3) evaluate correlations between the ncRNAs. METHODS: The study included 111 Cr(VI) exposed workers and 72 controls recruited from the SafeChrom project. Cr concentrations were measured in RBC (biomarker of long-term exposure) and urine (biomarker of short-term exposure) samples. Long ncRNA (lncRNA) and microRNA (miRNA) were extracted from plasma followed by deoxyribonuclease treatment, complementary DNA synthesis, and quantitative real-time polymerase chain reaction using target-specific assays for three lncRNAs (H19, MALAT1, NORAD), and four miRNAs (miR-142-3p, miR-15b-5p, miR-3940-5p, miR-451a). RESULTS: Expression levels of lncRNAs MALAT1 and NORAD, and all four miRNAs, were significantly lower in Cr(VI) exposed workers compared with controls, and correlated significantly with RBC-Cr concentrations (rS = -0.16 to -0.38). H19 was non-significantly increased in exposed workers but significantly correlated with miR-142-3p (rS = -0.33) and miR-15b-5p (rS = -0.30), and NORAD was significantly positively correlated with all four miRNAs (rS = 0.17 to 0.46). In multivariate regression models adjusting for confounders, expressions of lncRNAs MALAT1 and NORAD and all miRNAs were still significantly lower in the exposed group compared with controls, and the expression decreased with increasing RBC-Cr concentrations. CONCLUSIONS: Cr(VI) exposure was inversely and in a dose-response manner associated with the expression of circulating non-coding RNA, which suggests ncRNAs as potential biomarkers for Cr(VI)-induced toxicity. Correlations between miRNAs and lncRNAs suggest that they participate in the same lncRNA-miRNA-messenger RNA regulatory axes, which may play important roles in Cr(VI) carcinogenesis. |
Full Text Finder 
Nájsť tento článok vo Web of Science