Interaction of metals with peroxidase-mediated luminol-enhanced chemiluminescence (PLmCL)
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| Title: | Interaction of metals with peroxidase-mediated luminol-enhanced chemiluminescence (PLmCL) |
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| Authors: | Coteur, Geoffroy, Dubois, Philippe |
| Source: | Luminescence, 19 (5 |
| Publisher Information: | 2004 |
| Document Type: | Electronic Resource |
| Abstract: | The peroxidase-mediated luminol-enhanced chemiluminescence (PLmCL) method has been used to study the in vitro effect of contaminants such as heavy metals on the reactive oxygen species production by immunocytes. We were interested to know whether metals could directly affect peroxidase-mediated luminescence, taking horseradish peroxidase (HRP) as a model enzyme, since this could contribute to the inhibition of immunocyte LmCL. Copper inhibited PLmCL in a dose-dependent manner, while cadmium, iron, silver and lead only partly decreased the signal in the concentration range tested. In contrast, zinc enhanced the signal at high concentrations. Eventually, chromium, mercury and aluminium did not affect PLmCL. It is suggested that these effects reflect the ability of the metals to interact with the active site of the peroxidase. These results demonstrate that such interactions have to be considered when interpreting the effects of metals on immunocytes using the LmCL method. Copyright © 2004 John Wiley & Sons, Ltd. IF: 1,297 info:eu-repo/semantics/published |
| Index Terms: | Biologie des milieux particuliers, Animals, Catalysis, Horseradish Peroxidase -- chemistry -- metabolism, Hydrogen Peroxide -- chemistry, Luminescent Measurements -- methods, Luminol -- chemistry, Metals, Heavy -- chemistry -- metabolism, Oxidation-Reduction, Peroxidase -- chemistry -- metabolism, Reactive Oxygen Species -- analysis -- metabolism, Biologie marine, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| Availability: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| Note: | 1 full-text file(s): application/pdf English |
| Other Numbers: | EQY oai:dipot.ulb.ac.be:2013/115832 uri/info:doi/10.1002/bio.784 uri/info:pmid/15449349 uri/info:scp/14844322301 local/VX-004777 803496047 |
| Contributing Source: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| Accession Number: | edsoai.ocn803496047 |
| Database: | OAIster |
Nájsť tento článok vo Web of Science | Abstract: | The peroxidase-mediated luminol-enhanced chemiluminescence (PLmCL) method has been used to study the in vitro effect of contaminants such as heavy metals on the reactive oxygen species production by immunocytes. We were interested to know whether metals could directly affect peroxidase-mediated luminescence, taking horseradish peroxidase (HRP) as a model enzyme, since this could contribute to the inhibition of immunocyte LmCL. Copper inhibited PLmCL in a dose-dependent manner, while cadmium, iron, silver and lead only partly decreased the signal in the concentration range tested. In contrast, zinc enhanced the signal at high concentrations. Eventually, chromium, mercury and aluminium did not affect PLmCL. It is suggested that these effects reflect the ability of the metals to interact with the active site of the peroxidase. These results demonstrate that such interactions have to be considered when interpreting the effects of metals on immunocytes using the LmCL method. Copyright © 2004 John Wiley & Sons, Ltd.<br />IF: 1,297<br />info:eu-repo/semantics/published |
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