Variations in plant metallothioneins: the heavy metal hyperaccumulator Thlaspi caerulescens as a study case
Uloženo v:
| Název: | Variations in plant metallothioneins: the heavy metal hyperaccumulator Thlaspi caerulescens as a study case |
|---|---|
| Autoři: | Roosens, Nancy, Leplae, Raphaël, Bernard, Catherine, Verbruggen, Nathalie |
| Zdroj: | Planta, 222 (4 |
| Informace o vydavateli: | 2005 |
| Druh dokumentu: | Electronic Resource |
| Abstrakt: | Plant metallothioneins (MTs) are extremely diverse and are thought to be involved in metal homeostasis or detoxification. Thlaspi caerulescens is a model Zn/Cd hyperaccumulator and thus constitutes an ideal system to study the variability of these MTs. Two T. caerulescens cDNAs (accession: 665511; accession: 665515), that are highly homologous to type 1 and type 2 Arabidopsis thaliana MTs, have been isolated using a functional screen for plant cDNAs that confer Cd tolerance to yeast. However, TcMT1 has a much shorter N-terminal domain than that of A. thaliana and so lacks Cys motifs conserved through all the plant MTs classified as type 1. A systematic search in plant databases allowed the detection of MT-related sequences. Sixty-four percent fulfil the criteria for MT classification described in Cobbett and Goldsbrough (2002) and further extend our knowledge about other conserved residues that might play an important role in plant MT structure. In addition, 34% of the total MT-related sequences cannot be classified strictly as they display modifications in the conserved residues according to the current plant MTs' classification. The significance of this variability in plant MT sequences is discussed. Functional complementation in yeast was used to assess whether these variations may alter the MTs' function in T. caerulescens. Regulation of the expression of MTs in T. caerulescens was also investigated. TcMT1 and TcMT2 display higher expression in T. caerulescens than in A. thaliana. Moreover, their differential expression patterns in organs and in response to metal exposure, suggest that the two types of MTs may have diverse roles and functions in T. caerulescens. © Springer-Verlag 2005. Journal Article Research Support, Non-U.S. Gov't SCOPUS: ar.j info:eu-repo/semantics/published |
| Témata: | Génétique des plantes, Physiologie des plantes vasculaires, Amino Acid Sequence, Arabidopsis -- genetics, Arabidopsis -- metabolism, Arabidopsis Proteins -- metabolism, Cadmium -- metabolism, Cloning, Molecular, Copper -- metabolism, Metallothionein -- classification, Metallothionein -- genetics, Metallothionein -- metabolism, Metals, Heavy -- metabolism, Molecular Sequence Data, Oxidative Stress, Phylogeny, Plant Proteins -- classification, Plant Proteins -- genetics, Plant Proteins -- metabolism, RNA, Messenger -- metabolism, Saccharomyces cerevisiae -- genetics, Saccharomyces cerevisiae -- metabolism, Sequence Alignment, Sequence Homology, Amino Acid, Thlaspi -- genetics, Thlaspi -- metabolism, Zinc -- metabolism, Cadmium, Heavy metal stress, Metal homeostasis, Metal hyperaccumulation, Metallothionein, Thlaspi caerulescens, info:eu-repo/semantics/article, info:ulb-repo/semantics/articlePeerReview, info:ulb-repo/semantics/openurl/article |
| URL: | |
| Dostupnost: | Open access content. Open access content 1 full-text file(s): info:eu-repo/semantics/restrictedAccess |
| Poznámka: | 1 full-text file(s): application/pdf English |
| Other Numbers: | EQY oai:dipot.ulb.ac.be:2013/57914 uri/info:doi/10.1007/s00425-005-0006-1 uri/info:pmid/16052319 uri/info:scp/27644589548 local/VX-006087 778635699 |
| Přispívající zdroj: | UNIVERSITE LIBRE DE BRUXELLES From OAIster®, provided by the OCLC Cooperative. |
| Přístupové číslo: | edsoai.ocn778635699 |
| Databáze: | OAIster |
Nájsť tento článok vo Web of Science | Abstrakt: | Plant metallothioneins (MTs) are extremely diverse and are thought to be involved in metal homeostasis or detoxification. Thlaspi caerulescens is a model Zn/Cd hyperaccumulator and thus constitutes an ideal system to study the variability of these MTs. Two T. caerulescens cDNAs (accession: 665511; accession: 665515), that are highly homologous to type 1 and type 2 Arabidopsis thaliana MTs, have been isolated using a functional screen for plant cDNAs that confer Cd tolerance to yeast. However, TcMT1 has a much shorter N-terminal domain than that of A. thaliana and so lacks Cys motifs conserved through all the plant MTs classified as type 1. A systematic search in plant databases allowed the detection of MT-related sequences. Sixty-four percent fulfil the criteria for MT classification described in Cobbett and Goldsbrough (2002) and further extend our knowledge about other conserved residues that might play an important role in plant MT structure. In addition, 34% of the total MT-related sequences cannot be classified strictly as they display modifications in the conserved residues according to the current plant MTs' classification. The significance of this variability in plant MT sequences is discussed. Functional complementation in yeast was used to assess whether these variations may alter the MTs' function in T. caerulescens. Regulation of the expression of MTs in T. caerulescens was also investigated. TcMT1 and TcMT2 display higher expression in T. caerulescens than in A. thaliana. Moreover, their differential expression patterns in organs and in response to metal exposure, suggest that the two types of MTs may have diverse roles and functions in T. caerulescens. © Springer-Verlag 2005.<br />Journal Article<br />Research Support, Non-U.S. Gov't<br />SCOPUS: ar.j<br />info:eu-repo/semantics/published |
|---|