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Efficient characterization of red blood cell rheological properties using a multichannel microfluidic chip and optical tweezers

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Názov: Efficient characterization of red blood cell rheological properties using a multichannel microfluidic chip and optical tweezers
Autori: Ying Liu, Hongtao Rao, Hongliang Zhang, Meng Wang, Yinglian Wu, Ying Wu, Caiqin Han, Changchun Yan, Le Zhang, Wei Chen, JingJing Wang
Zdroj: Materials Today Advances, Vol 24, Iss , Pp 100545- (2024)
Informácie o vydavateľovi: Elsevier, 2024.
Rok vydania: 2024
Zbierka: LCC:Materials of engineering and construction. Mechanics of materials
Predmety: Rheology, Red blood cell, Microfluid, Optical tweezer, Materials of engineering and construction. Mechanics of materials, TA401-492
Popis: The rheological properties of red blood cells (RBCs) are crucial for human health. Combining optical tweezers with microfluidics provides a non-contact, sensitive, and high-throughput method for studying RBC rheology. However, the limited trapping capacity of optical tweezers restricts RBC flow within microchannels, reducing individual RBC capture efficiency. To address this, we developed a multichannel microfluidic chip with a pressure relief structure. Integrating this with optical tweezers using time-division multiplexing enabled simultaneous capture of RBCs across multiple microchannels. This method not only enhances sample throughput during optical tweezer measurements but also allows individual capture and analysis of multiple flowing RBCs in the same timeframe. Image recognition analysis of RBCs captured by optical tweezers revealed distinct morphological differences between normal and diseased RBCs, consistent with finite element method simulations of RBC rheological behavior. This approach provides quantitative characterization of RBC rheology and enables effective detection.
Druh dokumentu: article
Popis súboru: electronic resource
Jazyk: English
ISSN: 2590-0498
Relation: http://www.sciencedirect.com/science/article/pii/S2590049824000821; https://doaj.org/toc/2590-0498
DOI: 10.1016/j.mtadv.2024.100545
Prístupová URL adresa: https://doaj.org/article/88097fbe8cf14ab2829df54c9d96a9b5
Prístupové číslo: edsdoj.88097fbe8cf14ab2829df54c9d96a9b5
Databáza: Directory of Open Access Journals
Popis
Abstrakt:The rheological properties of red blood cells (RBCs) are crucial for human health. Combining optical tweezers with microfluidics provides a non-contact, sensitive, and high-throughput method for studying RBC rheology. However, the limited trapping capacity of optical tweezers restricts RBC flow within microchannels, reducing individual RBC capture efficiency. To address this, we developed a multichannel microfluidic chip with a pressure relief structure. Integrating this with optical tweezers using time-division multiplexing enabled simultaneous capture of RBCs across multiple microchannels. This method not only enhances sample throughput during optical tweezer measurements but also allows individual capture and analysis of multiple flowing RBCs in the same timeframe. Image recognition analysis of RBCs captured by optical tweezers revealed distinct morphological differences between normal and diseased RBCs, consistent with finite element method simulations of RBC rheological behavior. This approach provides quantitative characterization of RBC rheology and enables effective detection.
ISSN:25900498
DOI:10.1016/j.mtadv.2024.100545