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Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study

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Název: Screening of Red Sea- and Mediterranean Sea-derived Actinomycetes for Antimicrobial and Antitumor activities: LC-ESI-HRMS-based Metabolomics Study
Autoři: Mahmoud A. Abdel-Razik, Ahmed F. Azmy, Tarek Dishisha, Ahmed O. El-Gendy, Adlin Afzan, Nurkhalida Kamal, Ahmed Tawfike, Mohamed Sebak
Zdroj: Microbial Cell Factories, Vol 24, Iss 1, Pp 1-21 (2025)
Informace o vydavateli: BMC, 2025.
Rok vydání: 2025
Sbírka: LCC:Microbiology
Témata: Antimicrobial, Cytotoxicity, Dereplication, LC-HRMS, Marine actinomycetes, Metabolomics, Microbiology, QR1-502
Popis: Abstract In reply to the critical requirement for new anti-infective and anticancer agents, this study explores aquatic actinomycetes as a favorable source of naturally occurring bioactive compounds. Using metabolomics and dereplication analysis, we investigated the chemical profiles of promising eight actinobacterial isolates out of seventy-eight actinomycetes isolated from the Red Sea and the Mediterranean Sea. The eight isolates (6, 10, 12, 35, 42, 43, 45, and 48) demonstrated cytotoxic activity against the breast adenocarcinoma (MCF-7) cell line and broad-spectrum antimicrobial activity against Gram-negative and Gram-positive microbes. Isolate 10 exhibited the broadest antibacterial activity, while isolates 42, 43, and 45 showed moderate to weak cytotoxic potential. Genetic identification via 16S rRNA sequencing classified seven isolates as Streptomyces sp. and one as Brevibacterium sp. Multivariate data analysis (MVDA) of the high-resolution mass spectrometry (HRMS) data identified isolate 10 as an outlier, rich in diverse secondary metabolites. Dereplication revealed numerous metabolites with potential antimicrobial and anticancer activity, confirmed by NMR data highlighting their complex chemical profiles. These findings underscore the effectiveness of integrating metabolomics and bioassays to uncover unique bioactive products from aquatic actinobacteria, creating a foundation for further development of novel antimicrobial and antitumor medicines.
Druh dokumentu: article
Popis souboru: electronic resource
Jazyk: English
ISSN: 1475-2859
Relation: https://doaj.org/toc/1475-2859
DOI: 10.1186/s12934-025-02759-0
Přístupová URL adresa: https://doaj.org/article/e7d74f7938a54ab9bef64b6f924bd877
Přístupové číslo: edsdoj.7d74f7938a54ab9bef64b6f924bd877
Databáze: Directory of Open Access Journals
Popis
Abstrakt:Abstract In reply to the critical requirement for new anti-infective and anticancer agents, this study explores aquatic actinomycetes as a favorable source of naturally occurring bioactive compounds. Using metabolomics and dereplication analysis, we investigated the chemical profiles of promising eight actinobacterial isolates out of seventy-eight actinomycetes isolated from the Red Sea and the Mediterranean Sea. The eight isolates (6, 10, 12, 35, 42, 43, 45, and 48) demonstrated cytotoxic activity against the breast adenocarcinoma (MCF-7) cell line and broad-spectrum antimicrobial activity against Gram-negative and Gram-positive microbes. Isolate 10 exhibited the broadest antibacterial activity, while isolates 42, 43, and 45 showed moderate to weak cytotoxic potential. Genetic identification via 16S rRNA sequencing classified seven isolates as Streptomyces sp. and one as Brevibacterium sp. Multivariate data analysis (MVDA) of the high-resolution mass spectrometry (HRMS) data identified isolate 10 as an outlier, rich in diverse secondary metabolites. Dereplication revealed numerous metabolites with potential antimicrobial and anticancer activity, confirmed by NMR data highlighting their complex chemical profiles. These findings underscore the effectiveness of integrating metabolomics and bioassays to uncover unique bioactive products from aquatic actinobacteria, creating a foundation for further development of novel antimicrobial and antitumor medicines.
ISSN:14752859
DOI:10.1186/s12934-025-02759-0