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Protective Effect of Chinese Cabbage Outer Leaves Soluble Dietary Fiber on Intestinal Barrier Damage in Mice

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Titel: Protective Effect of Chinese Cabbage Outer Leaves Soluble Dietary Fiber on Intestinal Barrier Damage in Mice
Autoren: Zepeng GAO, Qian ZHOU, Jianjun ZHAO, Peng QIN, Yu CAO, Jiaqi ZHANG, Xinying CHENG, Wen ZHAO
Quelle: Shipin gongye ke-ji, Vol 46, Iss 19, Pp 408-421 (2025)
Verlagsinformationen: The editorial department of Science and Technology of Food Industry, 2025.
Publikationsjahr: 2025
Bestand: LCC:Food processing and manufacture
Schlagwörter: chinese cabbage outer leaf, soluble dietary fiber, cyclophosphamide, intestinal barrier, gut microbiota, Food processing and manufacture, TP368-456
Beschreibung: Objective: The intervention effect of soluble dietary fiber from the outer leaves of Chinese cabbage (CSF) on cyclophosphamide (CPA) induced intestinal barrier damage in mice were studied to provide a theoretical basis for the high-value utilization of CSF resources. Methods: Sixty male ICR mice were randomly divided into five groups: Negative control, model control, and 125, 250, and 500 mg/kg·bw CSF interventions. Structure of the intestinal epithelial tissue, serum and intestinal biochemical indices, tight binding proteins, short chain fatty acids (SCFAs), and intestinal flora were evaluated to determine the protective effect of CSF on intestinal barrier injury in mice. Results: CSF improved the integrity and arrangement of the villi and villus length in the small intestine, as well as the ratio of villus length to crypt depth. The serum levels of lipopolysaccharides (LPS), D-lactate acid (D-LA), and diamine oxidase (DAO) and intestinal permeability were reduced. The expression of zonula occludens-1 (ZO-1), Claudin-1, and Occludin proteins in the small intestinal tissue of model control mice was significantly reversed. The number of goblet cells and intraepithelial lymphocytes in jejunum and ileum were increased, and the expression level of SIgA, β-DF, and LZM were up-regulated. 16S rDNA sequencing was performed to detect the intestinal flora of mice, and it was found that CSF effectively restored the species diversity of the intestinal flora and had a significant regulatory effect on the structure and composition of the intestinal flora. In addition, SCFAs and total SCFAs in the CSF-treated group were significantly or extremely significantly restored. Conclusion: CSF exhibits potential protective effects against CPA-induced intestinal barrier injury in mice through physical, immune, and biological intestinal barriers.
Publikationsart: article
Dateibeschreibung: electronic resource
Sprache: Chinese
ISSN: 1002-0306
Relation: https://doaj.org/toc/1002-0306
DOI: 10.13386/j.issn1002-0306.2024110124
Zugangs-URL: https://doaj.org/article/c70eb7d7a50d477985b4ab80a2f3d013
Dokumentencode: edsdoj.70eb7d7a50d477985b4ab80a2f3d013
Datenbank: Directory of Open Access Journals
Beschreibung
Abstract:Objective: The intervention effect of soluble dietary fiber from the outer leaves of Chinese cabbage (CSF) on cyclophosphamide (CPA) induced intestinal barrier damage in mice were studied to provide a theoretical basis for the high-value utilization of CSF resources. Methods: Sixty male ICR mice were randomly divided into five groups: Negative control, model control, and 125, 250, and 500 mg/kg·bw CSF interventions. Structure of the intestinal epithelial tissue, serum and intestinal biochemical indices, tight binding proteins, short chain fatty acids (SCFAs), and intestinal flora were evaluated to determine the protective effect of CSF on intestinal barrier injury in mice. Results: CSF improved the integrity and arrangement of the villi and villus length in the small intestine, as well as the ratio of villus length to crypt depth. The serum levels of lipopolysaccharides (LPS), D-lactate acid (D-LA), and diamine oxidase (DAO) and intestinal permeability were reduced. The expression of zonula occludens-1 (ZO-1), Claudin-1, and Occludin proteins in the small intestinal tissue of model control mice was significantly reversed. The number of goblet cells and intraepithelial lymphocytes in jejunum and ileum were increased, and the expression level of SIgA, β-DF, and LZM were up-regulated. 16S rDNA sequencing was performed to detect the intestinal flora of mice, and it was found that CSF effectively restored the species diversity of the intestinal flora and had a significant regulatory effect on the structure and composition of the intestinal flora. In addition, SCFAs and total SCFAs in the CSF-treated group were significantly or extremely significantly restored. Conclusion: CSF exhibits potential protective effects against CPA-induced intestinal barrier injury in mice through physical, immune, and biological intestinal barriers.
ISSN:10020306
DOI:10.13386/j.issn1002-0306.2024110124