Breeding of high-temperature resistant Phaffia rhodozyma by compound mutation and fermentation condition optimization
Uloženo v:
| Název: | Breeding of high-temperature resistant Phaffia rhodozyma by compound mutation and fermentation condition optimization |
|---|---|
| Autoři: | ZHOU Guixiong, TAN Leitao, MA Ronghua, LIU Qiuping, FANG Zheng, WU Qingshan, XIANG Lan, WENG Qingbei |
| Zdroj: | Zhongguo niangzao, Vol 44, Iss 8, Pp 256-262 (2025) |
| Informace o vydavateli: | Editorial Department of China Brewing, 2025. |
| Rok vydání: | 2025 |
| Sbírka: | LCC:Biotechnology LCC:Food processing and manufacture |
| Témata: | phaffia rhodozyma, astaxanthin, compound mutation, response surface method, Biotechnology, TP248.13-248.65, Food processing and manufacture, TP368-456 |
| Popis: | To obtain a high-temperature-resistant astaxanthin-producing strain, using Phaffia rhodozyma AS2.1557 as the starting strain, the mutant strain was screened by multiple rounds of composite mutagenesis with ultraviolet (UV) light and ethyl methylsulfonate (EMS) in combination with high-temperature stress. The genetic stability of the mutant strain was verified through continuous transfer fermentation tests, and the fermentation conditions of the mutant strain were optimized by single factor tests and response surface tests. The results showed that a mutant strain UE-09 was obtained, which could grow normally and produce astaxanthin at 35 ℃. The mutant strain still had good genetic stability after eight subcultures. The optimal fermentation conditions for astaxanthin production were as follows: fermentation temperature 32 ℃, initial pH 5.5, inoculum 7.0%, and rotation speed 220 r/min. Under this optimal condition, the astaxanthin yield of this mutant strain reached up to 2.74 mg/L, which was 66.06% higher than that before optimization. This research would provide strain resources and theoretical guidance for the production of astaxanthin under high-temperature conditions. |
| Druh dokumentu: | article |
| Popis souboru: | electronic resource |
| Jazyk: | English Chinese |
| ISSN: | 0254-5071 |
| Relation: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-8-256.pdf; https://doaj.org/toc/0254-5071 |
| DOI: | 10.11882/j.issn.0254-5071.2025.08.037 |
| Přístupová URL adresa: | https://doaj.org/article/da491f73c7384e1aa62cb1b9e040f3a2 |
| Přístupové číslo: | edsdoj.491f73c7384e1aa62cb1b9e040f3a2 |
| Databáze: | Directory of Open Access Journals |
Nájsť tento článok vo Web of Science | Abstrakt: | To obtain a high-temperature-resistant astaxanthin-producing strain, using Phaffia rhodozyma AS2.1557 as the starting strain, the mutant strain was screened by multiple rounds of composite mutagenesis with ultraviolet (UV) light and ethyl methylsulfonate (EMS) in combination with high-temperature stress. The genetic stability of the mutant strain was verified through continuous transfer fermentation tests, and the fermentation conditions of the mutant strain were optimized by single factor tests and response surface tests. The results showed that a mutant strain UE-09 was obtained, which could grow normally and produce astaxanthin at 35 ℃. The mutant strain still had good genetic stability after eight subcultures. The optimal fermentation conditions for astaxanthin production were as follows: fermentation temperature 32 ℃, initial pH 5.5, inoculum 7.0%, and rotation speed 220 r/min. Under this optimal condition, the astaxanthin yield of this mutant strain reached up to 2.74 mg/L, which was 66.06% higher than that before optimization. This research would provide strain resources and theoretical guidance for the production of astaxanthin under high-temperature conditions. |
|---|---|
| ISSN: | 02545071 |
| DOI: | 10.11882/j.issn.0254-5071.2025.08.037 |