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Integrated multi-omics of gut-liver axis to dissect the mechanism underlying hepatotoxicity induced by sub-chronic tire wear particles exposure in mice

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Bibliographic Details
Title: Integrated multi-omics of gut-liver axis to dissect the mechanism underlying hepatotoxicity induced by sub-chronic tire wear particles exposure in mice
Authors: Yan Zhou, Pengfei Cheng, Jiahui Ran, Xin Li, Xia Yan, Lei Luo, Ming Xie, Kunming Tian
Source: Ecotoxicology and Environmental Safety, Vol 306, Iss , Pp 119350- (2025)
Publisher Information: Elsevier, 2025.
Publication Year: 2025
Collection: LCC:Environmental pollution
LCC:Environmental sciences
Subject Terms: Tire wear particles, Hepatotoxicity, Gut microbiota, Gut-liver axis, Environmental pollution, TD172-193.5, Environmental sciences, GE1-350
Description: As a novel pollutant, tire wear particles (TWPs) have been revealed as a major constituent of microplastics (MPs) and are widely present in various environmental media. Yet the current studies are limited to investigating their environmental distribution and aquatic toxicity, and the potential adverse effects of TWPs on human remain unclear. The mechanism underlying hepatotoxicity induced by long-term TWPs exposure has not yet been investigated. To address this issue, we conducted an animal experiment using forty 4-week-old female Balb/c mice, which were randomly divided into four groups (Control, L-TWPs, M-TWPs and H-TWPs). They were gavaged with PBS, 0.1 mg/day, 0.5 mg/day and 1 mg/day TWPs for 14 weeks, respectively. Results demonstrated that chronic TWPs exposure pathologically damaged liver, gut barrier, as well as induced gut microbiota dysbiosis. In addition, both the ileal and hepatic metabolomes indicated that TWPs caused riboflavin metabolism disorder. Furthermore, we found elevated abundance of Staphylococcus spp. was negatively correlated with reduced concentrations of glycerophosphoinositol, serotonin and glutathione that are responsible for maintaining intestinal barrier in ileum, while reduced Oligella spp. and Paenalcaligenes spp. were positively correlated with diminished levels of hepatic riboflavin, flavin mononucleotide and lumiflavin, all of which could maintain normal riboflavin metabolism. Consequently, TWPs-induced gut microbiota dysbiosis exacerbated intestinal barrier impairment, contributing to hepatic riboflavin metabolism disorder. In conclusion, our work provides novel evidence for deepening insight into long-term TWPs exposure-induced hepatotoxicity from the perspective of gut-liver axis.
Document Type: article
File Description: electronic resource
Language: English
ISSN: 0147-6513
Relation: http://www.sciencedirect.com/science/article/pii/S0147651325016951; https://doaj.org/toc/0147-6513
DOI: 10.1016/j.ecoenv.2025.119350
Access URL: https://doaj.org/article/ac41399bbb724b9ba6ad84bebe49589f
Accession Number: edsdoj.41399bbb724b9ba6ad84bebe49589f
Database: Directory of Open Access Journals
Description
Abstract:As a novel pollutant, tire wear particles (TWPs) have been revealed as a major constituent of microplastics (MPs) and are widely present in various environmental media. Yet the current studies are limited to investigating their environmental distribution and aquatic toxicity, and the potential adverse effects of TWPs on human remain unclear. The mechanism underlying hepatotoxicity induced by long-term TWPs exposure has not yet been investigated. To address this issue, we conducted an animal experiment using forty 4-week-old female Balb/c mice, which were randomly divided into four groups (Control, L-TWPs, M-TWPs and H-TWPs). They were gavaged with PBS, 0.1 mg/day, 0.5 mg/day and 1 mg/day TWPs for 14 weeks, respectively. Results demonstrated that chronic TWPs exposure pathologically damaged liver, gut barrier, as well as induced gut microbiota dysbiosis. In addition, both the ileal and hepatic metabolomes indicated that TWPs caused riboflavin metabolism disorder. Furthermore, we found elevated abundance of Staphylococcus spp. was negatively correlated with reduced concentrations of glycerophosphoinositol, serotonin and glutathione that are responsible for maintaining intestinal barrier in ileum, while reduced Oligella spp. and Paenalcaligenes spp. were positively correlated with diminished levels of hepatic riboflavin, flavin mononucleotide and lumiflavin, all of which could maintain normal riboflavin metabolism. Consequently, TWPs-induced gut microbiota dysbiosis exacerbated intestinal barrier impairment, contributing to hepatic riboflavin metabolism disorder. In conclusion, our work provides novel evidence for deepening insight into long-term TWPs exposure-induced hepatotoxicity from the perspective of gut-liver axis.
ISSN:01476513
DOI:10.1016/j.ecoenv.2025.119350