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A confining mesh enables concurrent calcium phosphate deposition and preservation of TiO2 nanotube topography for synergistic osteogenic regulation

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Titel: A confining mesh enables concurrent calcium phosphate deposition and preservation of TiO2 nanotube topography for synergistic osteogenic regulation
Autoren: Xiaoran Yu, Zhengchuan Zhang, Yang Yang, Yun Liu, Yiming Li, Feilong Deng, Ruogu Xu
Quelle: Materials & Design, Vol 260, Iss , Pp 115117- (2025)
Verlagsinformationen: Elsevier, 2025.
Publikationsjahr: 2025
Bestand: LCC:Materials of engineering and construction. Mechanics of materials
Schlagwörter: Topography, Bioactive coating deposition, Synergistic effect, Custom-made titanium bone substitutes, Accelerate osseointegration, Materials of engineering and construction. Mechanics of materials, TA401-492
Beschreibung: Bioactive coating would compromise the pre-engineered surface topography of custom-made titanium bone substitutes, diminishing their therapeutic synergy. Although titanium dioxide nanotubes (TNTs) serve as well-established platforms for molecular loading, post-deposition structural alterations frequently occur, compromising their functionality. Thus, we proposed a topography-preserving mesh strategy to maintain TNTs during coating processes. This ultrathin mesh adheres robustly to TNTs, enabling site-specific localized coatings. Using an integrated anodization-sonication protocol, we successfully fabricated the mesh and achieved spatially confined calcium phosphate (CaP) deposition while preserving TNTs, verified by scanning electron microscopy. Comparative evaluations were conducted: in vitro with preosteoblasts (MC3T3-E1) and macrophages (RAW 264.7), and in vivo using beagle mandibular implantation models, comparing CaP/Mesh/TNTs versus CaP/TNTs with compromised nanotopography. The CaP/Mesh/TNTs construct significantly enhanced MC3T3-E1 cell adhesion, proliferation, and osteogenic differentiation. Simultaneously, it induced pro-healing morphological adaptations in RAW 264.7 cells, promoting M2 polarization with upregulation of VEGF and PDGF-BB secretion. Furthermore, macrophage-conditioned medium from the CaP/Mesh/TNTs most potently enhanced MC3T3-E1 osteogenesis. In vivo validation confirmed accelerated early-phase osseointegration with CaP/Mesh/TNTs. This study demonstrates that loading approaches on nanostructured surfaces must address topographical degradation and provides a practical methodology for concurrent surface functionalization with preserved nanotopography.
Publikationsart: article
Dateibeschreibung: electronic resource
Sprache: English
ISSN: 0264-1275
Relation: http://www.sciencedirect.com/science/article/pii/S0264127525015382; https://doaj.org/toc/0264-1275
DOI: 10.1016/j.matdes.2025.115117
Zugangs-URL: https://doaj.org/article/2e3dc2ab5f0b40f4a403cd258aa9320b
Dokumentencode: edsdoj.2e3dc2ab5f0b40f4a403cd258aa9320b
Datenbank: Directory of Open Access Journals
Beschreibung
Abstract:Bioactive coating would compromise the pre-engineered surface topography of custom-made titanium bone substitutes, diminishing their therapeutic synergy. Although titanium dioxide nanotubes (TNTs) serve as well-established platforms for molecular loading, post-deposition structural alterations frequently occur, compromising their functionality. Thus, we proposed a topography-preserving mesh strategy to maintain TNTs during coating processes. This ultrathin mesh adheres robustly to TNTs, enabling site-specific localized coatings. Using an integrated anodization-sonication protocol, we successfully fabricated the mesh and achieved spatially confined calcium phosphate (CaP) deposition while preserving TNTs, verified by scanning electron microscopy. Comparative evaluations were conducted: in vitro with preosteoblasts (MC3T3-E1) and macrophages (RAW 264.7), and in vivo using beagle mandibular implantation models, comparing CaP/Mesh/TNTs versus CaP/TNTs with compromised nanotopography. The CaP/Mesh/TNTs construct significantly enhanced MC3T3-E1 cell adhesion, proliferation, and osteogenic differentiation. Simultaneously, it induced pro-healing morphological adaptations in RAW 264.7 cells, promoting M2 polarization with upregulation of VEGF and PDGF-BB secretion. Furthermore, macrophage-conditioned medium from the CaP/Mesh/TNTs most potently enhanced MC3T3-E1 osteogenesis. In vivo validation confirmed accelerated early-phase osseointegration with CaP/Mesh/TNTs. This study demonstrates that loading approaches on nanostructured surfaces must address topographical degradation and provides a practical methodology for concurrent surface functionalization with preserved nanotopography.
ISSN:02641275
DOI:10.1016/j.matdes.2025.115117