In vivo versus in vitro sperm selection
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| Názov: | In vivo versus in vitro sperm selection |
|---|---|
| Autori: | Michal Ješeta, Jana Antalíková, Adéla Doubravská, Lenka Mekiňová, Bartosz Kempisty, Pavel Ventruba, Igor Crha |
| Zdroj: | Journal of Men's Health, Vol 21, Iss 2, Pp 1-10 (2025) |
| Informácie o vydavateľovi: | MRE Press, 2025. |
| Rok vydania: | 2025 |
| Zbierka: | LCC:Medicine (General) |
| Predmety: | spermatozoa, ivf, sperm separation, icsi, microfluid separation, thermotaxis, chemotaxis, Medicine (General), R5-920 |
| Popis: | The separation of human spermatozoa is an important step in therapy of human infertility. Given that male fertility is decreasing and, as a result, fertilization techniques based on microinjection of sperm into the cytoplasm are being used more intensively, this question is becoming increasingly relevant. In recent years, microfluidic sperm processing techniques have been increasingly used. These methods are simple and easy to use, however, the question is to what extent they select the correct sperm. They are essentially based mainly on motility and do not reflect other navigational approaches such as chemotaxis, thermotaxis or rheotaxis. This review compares traditional, advances and novel in vitro methods of sperm separation which are commonly used during human infertility therapy in context of in vivo sperm separation in female reproductive system. |
| Druh dokumentu: | article |
| Popis súboru: | electronic resource |
| Jazyk: | English |
| ISSN: | 1875-6867 1875-6859 |
| Relation: | https://oss.jomh.org/files/article/20250228-502/pdf/JOMH2024101802.pdf; https://doaj.org/toc/1875-6867; https://doaj.org/toc/1875-6859 |
| DOI: | 10.22514/jomh.2025.016 |
| Prístupová URL adresa: | https://doaj.org/article/00a3e47258b84a45a464b1b024c8d795 |
| Prístupové číslo: | edsdoj.00a3e47258b84a45a464b1b024c8d795 |
| Databáza: | Directory of Open Access Journals |
Nájsť tento článok vo Web of Science | Abstrakt: | The separation of human spermatozoa is an important step in therapy of human infertility. Given that male fertility is decreasing and, as a result, fertilization techniques based on microinjection of sperm into the cytoplasm are being used more intensively, this question is becoming increasingly relevant. In recent years, microfluidic sperm processing techniques have been increasingly used. These methods are simple and easy to use, however, the question is to what extent they select the correct sperm. They are essentially based mainly on motility and do not reflect other navigational approaches such as chemotaxis, thermotaxis or rheotaxis. This review compares traditional, advances and novel in vitro methods of sperm separation which are commonly used during human infertility therapy in context of in vivo sperm separation in female reproductive system. |
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| ISSN: | 18756867 18756859 |
| DOI: | 10.22514/jomh.2025.016 |