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Mesoporous Silica particles are phagocytosed by microglia and induce a mild inflammatory response in vitro.

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Bibliographic Details
Title: Mesoporous Silica particles are phagocytosed by microglia and induce a mild inflammatory response in vitro.
Authors: Sala Jarque, Julia, García Lara, Elisa, Carreras Domínguez, Paula, Zhou, Chunfang, Rabaneda Lombarte, Neus, Solà i Subirana, Carme, Vidal Taboada, José Manuel, Feiler, Adam, Abrahamsson, Ninnie, Kozlova, Elena N., Saura Martí, Josep
Source: Articles publicats en revistes (Ciències Fisiològiques)
Publisher Information: Future Medicine
Publication Year: 2022
Collection: Dipòsit Digital de la Universitat de Barcelona
Subject Terms: Astròcits, Partícules (Matèria), Sílice, Inflamació, Micròglia, Circuit neuronal, Astrocytes, Particles, Silica, Inflammation, Microglia, Neural circuitry
Description: Aim: Mesoporous silica particles (MSPs) are broadly used drug delivery carriers. In this study, the authors analyzed the responses to MSPs of astrocytes and microglia, the two main cellular players in neuroinflammation. Materials & methods: Primary murine cortical mixed glial cultures were treated with rhodamine B-labeled MSPs. Results: MSPs are avidly internalized by microglial cells and remain inside the cells for at least 14 days. Despite this, MSPs do not affect glial cell viability or morphology, basal metabolic activity or oxidative stress. MSPs also do not affect mRNA levels of key proinflammatory genes; however, in combination with lipopolysaccharide, they significantly increase extracellular IL-1β levels. Conclusion: These results suggest that MSPs could be novel tools for specific drug delivery to microglial cells.
Document Type: article in journal/newspaper
File Description: 37 p.; application/pdf
Language: English
Relation: Versió postprint del document publicat a: https://doi.org/10.2217/nnm-2022-0026; Nanomedicine, 2022, vol. 17, num.15, p. 1077-1094; https://doi.org/10.2217/nnm-2022-0026; https://hdl.handle.net/2445/220243; 731295
Availability: https://hdl.handle.net/2445/220243
Rights: (c) Future Medicine, 2022 ; info:eu-repo/semantics/openAccess
Accession Number: edsbas.FCE6B5D9
Database: BASE
Description
Abstract:Aim: Mesoporous silica particles (MSPs) are broadly used drug delivery carriers. In this study, the authors analyzed the responses to MSPs of astrocytes and microglia, the two main cellular players in neuroinflammation. Materials & methods: Primary murine cortical mixed glial cultures were treated with rhodamine B-labeled MSPs. Results: MSPs are avidly internalized by microglial cells and remain inside the cells for at least 14 days. Despite this, MSPs do not affect glial cell viability or morphology, basal metabolic activity or oxidative stress. MSPs also do not affect mRNA levels of key proinflammatory genes; however, in combination with lipopolysaccharide, they significantly increase extracellular IL-1β levels. Conclusion: These results suggest that MSPs could be novel tools for specific drug delivery to microglial cells.