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Elution Profiles of Eleven PAHs from a C-18 Chromatographic Column by using a HPLC-DAD Method

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Title: Elution Profiles of Eleven PAHs from a C-18 Chromatographic Column by using a HPLC-DAD Method
Authors: Onchoke, Kefa Karimu, Chaudhry, Saad N, Dewell, Madilyn
Source: Faculty Publications
Publisher Information: SFA ScholarWorks
Publication Year: 2024
Collection: Stephen F. Austin State University, Texas: Scholar Works @ SFA
Subject Terms: Laboratories and Demonstrations, organic chemistry, Nitrated PAHs, PAHs, HPLC, dipole moments, density functional Theory (DFT), Analytical Chemistry, Chemistry, Computational Chemistry, Curriculum and Instruction, Curriculum and Social Inquiry, Environmental Chemistry, Scholarship of Teaching and Learning, Science and Mathematics Education
Description: In this article we present elution profiles of polycyclic aromatic hydrocarbons (PAHs) benzanthrone (BA), 2-nitrobenzanthrone (2-NBA), fluoranthene (FL), 3-nitrofluoranthene (3-NFL), pyrene (PY), 1-aminopyrene (1-APY), 1-nitropyrene (1-NPY), chrysene (CHY), 6-aminochrysene (6-ACHY), 6-nitrochrysene (6-NCHY) and triphenylene (TP) by using high performance liquid chromatography (HPLC). The separation was achieved via running an isocratic mobile phase solvent system (acetonitrile: methanol 50%:50%, v/v), and a flow rate of 0.75 mL/min. Two wavelengths (l = 280 nm and 254 nm) were monitored with a diode array detector (DAD). In addition, this study presents the calculated dipole moments via the . After optimization with use of an isocratic solvent system bands eluted from a C-18 column in the order: 1-APY (4.57 mins) < 6-ACHY (4.62 mins) < 2-NBA (5.77 min) < 6-NCHY (6.16) < 3-NFL (6.33 min) < BA (6.41min) < 1-NPY (6.45 min) < FL (6.47 mins) < PY (7.13 min) < TP (7.46 mins) < chrysene (7.62 mins). Theoretically calculated dipole moments (Table 2) are predictive of the retention times. They approximately are inversely correlated to the elution profiles; namely, pyrene (0.100 D) = chrysene (0.000 D) = triphenylene (0.0000 D) < fluoranthene (0.3506 D) < 6-aminochrysene (1.6856 D) < 2-NBA (2.0087 D) < 1-aminopyrene (2.0294 D) < benzanthrone (3.8516 D) < 6-nitrochrysene (5.3595 D) < 3-NFL (5.7965 D) < 1-NPY (5.9913 D).
Document Type: text
Language: unknown
Relation: https://scholarworks.sfasu.edu/chemistry_facultypubs/106; http://chemeducator.org/bibs/0029001/29240005.html
DOI: 10.1333/s00897242990a
Availability: https://scholarworks.sfasu.edu/chemistry_facultypubs/106
https://doi.org/10.1333/s00897242990a
http://chemeducator.org/bibs/0029001/29240005.html
Accession Number: edsbas.FA4200A8
Database: BASE
Description
Abstract:In this article we present elution profiles of polycyclic aromatic hydrocarbons (PAHs) benzanthrone (BA), 2-nitrobenzanthrone (2-NBA), fluoranthene (FL), 3-nitrofluoranthene (3-NFL), pyrene (PY), 1-aminopyrene (1-APY), 1-nitropyrene (1-NPY), chrysene (CHY), 6-aminochrysene (6-ACHY), 6-nitrochrysene (6-NCHY) and triphenylene (TP) by using high performance liquid chromatography (HPLC). The separation was achieved via running an isocratic mobile phase solvent system (acetonitrile: methanol 50%:50%, v/v), and a flow rate of 0.75 mL/min. Two wavelengths (l = 280 nm and 254 nm) were monitored with a diode array detector (DAD). In addition, this study presents the calculated dipole moments via the . After optimization with use of an isocratic solvent system bands eluted from a C-18 column in the order: 1-APY (4.57 mins) < 6-ACHY (4.62 mins) < 2-NBA (5.77 min) < 6-NCHY (6.16) < 3-NFL (6.33 min) < BA (6.41min) < 1-NPY (6.45 min) < FL (6.47 mins) < PY (7.13 min) < TP (7.46 mins) < chrysene (7.62 mins). Theoretically calculated dipole moments (Table 2) are predictive of the retention times. They approximately are inversely correlated to the elution profiles; namely, pyrene (0.100 D) = chrysene (0.000 D) = triphenylene (0.0000 D) < fluoranthene (0.3506 D) < 6-aminochrysene (1.6856 D) < 2-NBA (2.0087 D) < 1-aminopyrene (2.0294 D) < benzanthrone (3.8516 D) < 6-nitrochrysene (5.3595 D) < 3-NFL (5.7965 D) < 1-NPY (5.9913 D).
DOI:10.1333/s00897242990a