When subcellular chemical imaging enlightens our understanding on intestinal absorption, intracellular fate and toxicity of PFOA in vitro.
Uloženo v:
| Název: | When subcellular chemical imaging enlightens our understanding on intestinal absorption, intracellular fate and toxicity of PFOA in vitro. |
|---|---|
| Autoři: | Stoffels, Charlotte, Cambier, Sébastien, Subirana, Maria A, Schaumlöffel, Dirk, Gomez, Gemma, Pittois, Denis, Guignard, Cédric, SCHWAMBORN, Jens Christian, WIRTZ, Tom, GUTLEB, Arno Christian, Mercier-Bonin, Muriel, AUDINOT, Jean-Nicolas |
| Zdroj: | Journal of Hazardous Materials, 480, 136205 (2024-12-05) |
| Informace o vydavateli: | Elsevier B.V. |
| Rok vydání: | 2024 |
| Sbírka: | University of Luxembourg: ORBilu - Open Repository and Bibliography |
| Témata: | Imaging, In vitro toxicology, Intestinal model, PFAS, SIMS, Fluorocarbons, perfluorooctanoic acid, Caprylates, Persistent Organic Pollutants, Environmental Pollutants, Humans, Caco-2 Cells, Oxidative Stress/drug effects, Cytosol/metabolism, Cytosol/drug effects, Persistent Organic Pollutants/toxicity, Persistent Organic Pollutants/metabolism, Cell Survival/drug effects, Environmental Pollutants/toxicity, Environmental Pollutants/pharmacokinetics, Fluorocarbons/toxicity, Fluorocarbons/pharmacokinetics, Caprylates/toxicity, Caprylates/pharmacokinetics, Intestinal Absorption/drug effects, Chemical imaging, In-vitro, Intestinal absorption, Organics, Sub-cellular |
| Popis: | peer reviewed ; Perfluorooctanoic acid (PFOA) is a persistent organic pollutant that accumulates in the human body, leading to major health issues. Upon oral uptake, the gastrointestinal tract is the first biological barrier against PFOA. However, the localization of PFOA and its impact on the intestinal wall are largely unknown. Here we achieve a breakthrough in the knowledge of intestinal absorption, intracellular fate and toxicity of PFOA using in vitro assays combined with novel analytical imaging techniques. For the first time, we localized PFOA in the cytosol of Caco-2 cells after acute exposure using high spatial resolution mass spectrometry imaging, and we estimated the PFOA cytosolic concentration. Knowing that PFOA enters and accumulates in the intestinal cells, we also performed common toxicity assays assessing cell metabolic activity, membrane integrity, oxidative stress response, and cell respiration. This study integrating powerful analytical techniques with widely used toxicology assays provides insightful information to better understand potential negative impacts of PFOA and opens new opportunities in toxicology and life science in general. |
| Druh dokumentu: | article in journal/newspaper |
| Jazyk: | English |
| ISSN: | 0304-3894 1873-3336 |
| Relation: | https://api.elsevier.com/content/article/PII:S0304389424027845?httpAccept=text/xml; urn:issn:0304-3894; urn:issn:1873-3336; https://orbilu.uni.lu/handle/10993/63445; info:hdl:10993/63445; https://orbilu.uni.lu/bitstream/10993/63445/1/2024_Stoffels.pdf; info:pmid:39454333; wos:001344459300001 |
| DOI: | 10.1016/j.jhazmat.2024.136205 |
| Dostupnost: | https://orbilu.uni.lu/handle/10993/63445 https://orbilu.uni.lu/bitstream/10993/63445/1/2024_Stoffels.pdf https://doi.org/10.1016/j.jhazmat.2024.136205 |
| Rights: | open access ; http://purl.org/coar/access_right/c_abf2 ; info:eu-repo/semantics/openAccess |
| Přístupové číslo: | edsbas.DFB9A54C |
| Databáze: | BASE |
Full Text Finder 
Nájsť tento článok vo Web of Science | Abstrakt: | peer reviewed ; Perfluorooctanoic acid (PFOA) is a persistent organic pollutant that accumulates in the human body, leading to major health issues. Upon oral uptake, the gastrointestinal tract is the first biological barrier against PFOA. However, the localization of PFOA and its impact on the intestinal wall are largely unknown. Here we achieve a breakthrough in the knowledge of intestinal absorption, intracellular fate and toxicity of PFOA using in vitro assays combined with novel analytical imaging techniques. For the first time, we localized PFOA in the cytosol of Caco-2 cells after acute exposure using high spatial resolution mass spectrometry imaging, and we estimated the PFOA cytosolic concentration. Knowing that PFOA enters and accumulates in the intestinal cells, we also performed common toxicity assays assessing cell metabolic activity, membrane integrity, oxidative stress response, and cell respiration. This study integrating powerful analytical techniques with widely used toxicology assays provides insightful information to better understand potential negative impacts of PFOA and opens new opportunities in toxicology and life science in general. |
|---|---|
| ISSN: | 03043894 18733336 |
| DOI: | 10.1016/j.jhazmat.2024.136205 |