Effects of juvenile hormone analogue insecticides, fenoxycarb and methoprene, on Neocaridina davidi and Moina macrocopa
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| Název: | Effects of juvenile hormone analogue insecticides, fenoxycarb and methoprene, on Neocaridina davidi and Moina macrocopa |
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| Přispěvatelé: | Hu, Xuelei (author.), Chu, Ka Hou (thesis advisor.), Chinese University of Hong Kong Graduate School. Division of Life Sciences. (degree granting institution.) |
| Rok vydání: | 2019 |
| Sbírka: | The Chinese University of Hong Kong: CUHK Digital Repository / 香港中文大學數碼典藏 |
| Témata: | Juvenile hormones--Agonists, Insecticides--Toxicology, Carbamates--Toxicology, Endocrine toxicology, Atyidae (Crustacea), Moina, Insecticides--toxicity, Phenylcarbamates--toxicity, Methoprene--toxicity, Decapoda, Arthropods, SF768.3 .H85 2019eb |
| Popis: | Ph.D. ; The increasing quantities of insecticides released into water bodies severely affect the health of the aquatic ecosystem. For instance, juvenile hormone analogue (JHA) insecticides are endocrine disrupters that interfere with hormonal action in insects by mimicking their juvenile hormones (JH). As the structure and functions of methyl farnesoate in crustaceans are similar to those of JH in insects, exogenous JHA insecticides could have adverse effects on the development and reproduction of crustaceans. Through phenotypic observation and transcriptome analysis, this study examined the toxic effects of two representative JHA insecticides, fenoxycarb and methoprene, on two freshwater crustacean models, Neocaridina davidi and Moina macrocopa. ; The results show that both insecticides had detrimental effects on cherry shrimp, but fenoxycarb was more toxic than methoprene. Chronic exposure to these insecticides reduced the shrimp’s body length and molting frequency. Based on transcriptome annotations for N. davidi, I identified important gene homologues that were active in both insect JH biosynthetic and degradative pathways as well as JH and ecdysteroid signaling pathways. Chronic treatments with JHAs had significant effects on these genes in N. davidi. RNA sequencing data showed that genes involved in the pathways related to cuticle development, serine protease activity, and carbohydrate, peptide and lipid metabolic processes were differentially expressed in shrimp exposed to JHAs. ; Results in M. macrocopa indicate that the 24-h and 48-h LC50 values for fenoxycarb and methoprene were 0.53 and 0.32 mg/L and 0.70 and 0.54 mg/L, respectively. Chronic exposures to fenoxycarb and methoprene caused a series of toxic effects in M. macrocopa; they shortened its life expectancy, repressed its body development, and reduced its fecundity. These JHAs also disturbed the expression of genes involved in the JH signaling pathway, in cuticle development, and in the carbohydrate, amino acid, and ATP metabolic processes. ... |
| Druh dokumentu: | text |
| Popis souboru: | electronic resource; remote; 1 online resource (xx, 136 leaves) : illustrations (some color); computer; online resource |
| Jazyk: | English Chinese |
| Relation: | cuhk:2398883; local: ETD920201144; local: AAI27784035; local: 991039875410103407 |
| Dostupnost: | https://julac.hosted.exlibrisgroup.com/primo-explore/search?query=addsrcrid,exact,991039875410103407,AND&tab=default_tab&search_scope=All&vid=CUHK&mode=advanced&lang=en_US https://repository.lib.cuhk.edu.hk/en/item/cuhk-2398883 |
| Rights: | Use of this resource is governed by the terms and conditions of the Creative Commons "Attribution-NonCommercial-NoDerivatives 4.0 International" License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
| Přístupové číslo: | edsbas.CE0BE56F |
| Databáze: | BASE |
Nájsť tento článok vo Web of Science | Abstrakt: | Ph.D. ; The increasing quantities of insecticides released into water bodies severely affect the health of the aquatic ecosystem. For instance, juvenile hormone analogue (JHA) insecticides are endocrine disrupters that interfere with hormonal action in insects by mimicking their juvenile hormones (JH). As the structure and functions of methyl farnesoate in crustaceans are similar to those of JH in insects, exogenous JHA insecticides could have adverse effects on the development and reproduction of crustaceans. Through phenotypic observation and transcriptome analysis, this study examined the toxic effects of two representative JHA insecticides, fenoxycarb and methoprene, on two freshwater crustacean models, Neocaridina davidi and Moina macrocopa. ; The results show that both insecticides had detrimental effects on cherry shrimp, but fenoxycarb was more toxic than methoprene. Chronic exposure to these insecticides reduced the shrimp’s body length and molting frequency. Based on transcriptome annotations for N. davidi, I identified important gene homologues that were active in both insect JH biosynthetic and degradative pathways as well as JH and ecdysteroid signaling pathways. Chronic treatments with JHAs had significant effects on these genes in N. davidi. RNA sequencing data showed that genes involved in the pathways related to cuticle development, serine protease activity, and carbohydrate, peptide and lipid metabolic processes were differentially expressed in shrimp exposed to JHAs. ; Results in M. macrocopa indicate that the 24-h and 48-h LC50 values for fenoxycarb and methoprene were 0.53 and 0.32 mg/L and 0.70 and 0.54 mg/L, respectively. Chronic exposures to fenoxycarb and methoprene caused a series of toxic effects in M. macrocopa; they shortened its life expectancy, repressed its body development, and reduced its fecundity. These JHAs also disturbed the expression of genes involved in the JH signaling pathway, in cuticle development, and in the carbohydrate, amino acid, and ATP metabolic processes. ... |
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