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Glutaraldehyde-based desensitizers' influence on bonding performances and dentin enzymatic activity of universal adhesives

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Název: Glutaraldehyde-based desensitizers' influence on bonding performances and dentin enzymatic activity of universal adhesives
Autoři: Mancuso, Edoardo, Durso, Diego, Mazzitelli, Claudia, Maravic, Tatjana, Josic, Uros, D'alessandro, Carlo, Generali, Luigi, Checchi, Vittorio, Breschi, Lorenzo, Mazzoni, Annalisa
Přispěvatelé: Mancuso, Edoardo, Durso, Diego, Mazzitelli, Claudia, Maravic, Tatjana, Josic, Uro, D'alessandro, Carlo, Generali, Luigi, Checchi, Vittorio, Breschi, Lorenzo, Mazzoni, Annalisa
Rok vydání: 2023
Sbírka: IRIS Università degli Studi di Bologna (CRIS - Current Research Information System)
Témata: Dentin, Desensitizer, Glutaraldehyde, Mmp inhibition, Universal adhesive, Zymography
Popis: Objectives: To evaluate the influence of two glutaraldehyde-based desensitizers (L: GLUMA Desensitizer, Heraeus Kulzer and G: GLUMA Desensitizer PowerGel) prior to the adhesive procedures on microtensile bond strength (& mu;TBS) to dentin and endogenous enzymatic activity. Methods: Noncarious human third molars (N = 48) were cut to expose middle coronal dentin. Six experimental groups were formed according to the dentin pre-treatment (L or G) and the universal adhesives (IBU -iBond universal, Kulzer or AU -Adhese Universal, Ivoclar Vivadent) used in the self-etch mode (n = 8): 1) L/IBU; 2) G/ IBU; 3) IBU; 4) L/AU; 5) G/AU; 6) AU. Specimens were cut into sticks and stressed until failure after 24 h (T0) or 1 yr of aging (T12). Additional 4 teeth were used for in situ zymography evaluation and data were statistically analyzed (& alpha; = 0.05). Results: Dentin pre-treatment, adhesive and aging statistically influenced bond strength and enzymatic activity (P<0.001). AU demonstrated higher bond strength values than IBU (P<0.001). The L resulted in higher bond strength compared to the G and control groups (P<0.001). aging statistically influenced bonding performance, especially when no dentin pre-treatment was performed (P<0.001). In situ zymography revealed that at baseline the control groups exhibited lower interfacial fluorescence compared to the experimental groups, irrespective of the adhesive used (P<0,001). However, after 1 yr of artificial storage, no differences were found among the groups (P>0.05). Conclusions: : Glutharldeadeyde-based products increased bond strength and determined a stabilization of the adhesive interface over time apparently not related to the MMPs inhibition. Clinical Significance: The results of this in vitro study suggest that the application of glutaraldehyde-based de-sensitizers prior to the adhesive procedures when associated with universal adhesives could result in increased bond strength and stabilization of the adhesive interface over time.
Druh dokumentu: article in journal/newspaper
Popis souboru: ELETTRONICO
Jazyk: English
Relation: info:eu-repo/semantics/altIdentifier/pmid/37524197; info:eu-repo/semantics/altIdentifier/wos/WOS:001051557600001; volume:136; firstpage:1; lastpage:10; numberofpages:10; journal:JOURNAL OF DENTISTRY; https://hdl.handle.net/11585/941214; https://www.sciencedirect.com/science/article/pii/S0300571223002294?via=ihub
DOI: 10.1016/j.jdent.2023.104643
Dostupnost: https://hdl.handle.net/11585/941214
https://doi.org/10.1016/j.jdent.2023.104643
https://www.sciencedirect.com/science/article/pii/S0300571223002294?via=ihub
Rights: info:eu-repo/semantics/openAccess
Přístupové číslo: edsbas.CAC61DF7
Databáze: BASE
Popis
Abstrakt:Objectives: To evaluate the influence of two glutaraldehyde-based desensitizers (L: GLUMA Desensitizer, Heraeus Kulzer and G: GLUMA Desensitizer PowerGel) prior to the adhesive procedures on microtensile bond strength (& mu;TBS) to dentin and endogenous enzymatic activity. Methods: Noncarious human third molars (N = 48) were cut to expose middle coronal dentin. Six experimental groups were formed according to the dentin pre-treatment (L or G) and the universal adhesives (IBU -iBond universal, Kulzer or AU -Adhese Universal, Ivoclar Vivadent) used in the self-etch mode (n = 8): 1) L/IBU; 2) G/ IBU; 3) IBU; 4) L/AU; 5) G/AU; 6) AU. Specimens were cut into sticks and stressed until failure after 24 h (T0) or 1 yr of aging (T12). Additional 4 teeth were used for in situ zymography evaluation and data were statistically analyzed (& alpha; = 0.05). Results: Dentin pre-treatment, adhesive and aging statistically influenced bond strength and enzymatic activity (P<0.001). AU demonstrated higher bond strength values than IBU (P<0.001). The L resulted in higher bond strength compared to the G and control groups (P<0.001). aging statistically influenced bonding performance, especially when no dentin pre-treatment was performed (P<0.001). In situ zymography revealed that at baseline the control groups exhibited lower interfacial fluorescence compared to the experimental groups, irrespective of the adhesive used (P<0,001). However, after 1 yr of artificial storage, no differences were found among the groups (P>0.05). Conclusions: : Glutharldeadeyde-based products increased bond strength and determined a stabilization of the adhesive interface over time apparently not related to the MMPs inhibition. Clinical Significance: The results of this in vitro study suggest that the application of glutaraldehyde-based de-sensitizers prior to the adhesive procedures when associated with universal adhesives could result in increased bond strength and stabilization of the adhesive interface over time.
DOI:10.1016/j.jdent.2023.104643