Synthesis and Molecular Characterization of Pyrene-Containing Copolymers as Potential MALDI-TOF MS Matrices
Saved in:
| Title: | Synthesis and Molecular Characterization of Pyrene-Containing Copolymers as Potential MALDI-TOF MS Matrices |
|---|---|
| Authors: | Marileta Tsakanika, Eleni Aleiferi, Dimitrios Damalas, Anastasia Stergiou, Nikolaos S. Thomaidis, Georgios Sakellariou |
| Publication Year: | 2025 |
| Subject Terms: | Biophysics, Biochemistry, Medicine, Cell Biology, Biotechnology, Inorganic Chemistry, Computational Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, vital analytical tool, prominent absorption capabilities, p3ddt ), highlighting, overcoming significant challenges, narrow dispersity (<, interfering background signals, g ., lipids, assisted laser desorption, analyte signal intensities, allowing precise control, novel linear polymers, mass spectrometric analysis, mass spectrometry, đ <, weight region, weight compounds, taking advantage, robust tools, reactivity ratios |
| Description: | Due to its exceptional sensitivity, accuracy, and speed, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has emerged as a vital analytical tool, especially for the determination of low-molecular-weight compounds (e.g., lipids, metabolites). Continuous advancements in MALDI-TOF technology have expanded its applications. The employment of polymeric materials as matrices has proven to be effective in overcoming significant challenges (self-ionization and adduct formation), particularly those related to interfering background signals in the low-molecular-weight region, making the development of effective matrices a critical area of research. We investigated the synthesis of well-defined polymers that meet the requirements of a suitable matrix for MALDI-TOF MS. In this study, pyrene was chosen as a chromophore to enhance the optical properties of the polymers, taking advantage of its aromatic structure and prominent absorption capabilities. We present the detailed synthesis of novel linear polymers through reversible addition–fragmentation chain transfer polymerization, which afforded macromolecules in a controlled manner and with narrow dispersity ( Đ ). Reactivity ratios were calculated to provide insight into the copolymerization behavior, allowing precise control over the polymer composition. Finally, these pyrene-incorporating polymers were tested and evaluated for their applicability as MALDI-TOF matrices, particularly in the analysis of low-molecular-weight compounds. Their performance was assessed based on analyte signal intensities and in comparison with other commercially available polymeric matrices (P3DDT), highlighting their potential as robust tools for mass spectrometric analysis. |
| Document Type: | article in journal/newspaper |
| Language: | unknown |
| DOI: | 10.1021/acs.macromol.5c00492.s001 |
| Availability: | https://doi.org/10.1021/acs.macromol.5c00492.s001 https://figshare.com/articles/journal_contribution/Synthesis_and_Molecular_Characterization_of_Pyrene-Containing_Copolymers_as_Potential_MALDI-TOF_MS_Matrices/29512439 |
| Rights: | CC BY-NC 4.0 |
| Accession Number: | edsbas.C41825AF |
| Database: | BASE |
Nájsť tento článok vo Web of Science | Abstract: | Due to its exceptional sensitivity, accuracy, and speed, matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has emerged as a vital analytical tool, especially for the determination of low-molecular-weight compounds (e.g., lipids, metabolites). Continuous advancements in MALDI-TOF technology have expanded its applications. The employment of polymeric materials as matrices has proven to be effective in overcoming significant challenges (self-ionization and adduct formation), particularly those related to interfering background signals in the low-molecular-weight region, making the development of effective matrices a critical area of research. We investigated the synthesis of well-defined polymers that meet the requirements of a suitable matrix for MALDI-TOF MS. In this study, pyrene was chosen as a chromophore to enhance the optical properties of the polymers, taking advantage of its aromatic structure and prominent absorption capabilities. We present the detailed synthesis of novel linear polymers through reversible addition–fragmentation chain transfer polymerization, which afforded macromolecules in a controlled manner and with narrow dispersity ( Đ ). Reactivity ratios were calculated to provide insight into the copolymerization behavior, allowing precise control over the polymer composition. Finally, these pyrene-incorporating polymers were tested and evaluated for their applicability as MALDI-TOF matrices, particularly in the analysis of low-molecular-weight compounds. Their performance was assessed based on analyte signal intensities and in comparison with other commercially available polymeric matrices (P3DDT), highlighting their potential as robust tools for mass spectrometric analysis. |
|---|---|
| DOI: | 10.1021/acs.macromol.5c00492.s001 |