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Calcium Signaling Dynamics in Migrating Epithelial Cells ; Kalciumsignalers dynamik i migrerande epitelceller

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Název: Calcium Signaling Dynamics in Migrating Epithelial Cells ; Kalciumsignalers dynamik i migrerande epitelceller
Autoři: Altuni, Bestun
Informace o vydavateli: KTH, Tillämpad fysik
Rok vydání: 2025
Sbírka: Royal Inst. of Technology, Stockholm (KTH): Publication Database DiVA
Témata: Calcium signaling, Gap junctions, Ouabain, Cx43, MDCK-II, GCaMP6m, Intercellular communication, Fluorescence microscopy, Signal correlation, Kalciumsignalering, Intercellulär kommunikation, Fluorescensmikroskopi, Signalkorrelation, Physical Sciences, Fysik
Popis: Intercellular calcium signaling plays a crucial role in coordinating epithelial cell behavior during tissue repair, development, and disease progression. Gap junctional intercellular communication (GJIC), primarily mediated by connexin proteins such as connexin 43 (Cx43), enables direct molecular exchange between adjacent cells. While previous studies have explored calcium signaling in MDCK-II epithelial cells and developed tools for visualizing intracellular calcium fluctuations, the specific effects of pharmacological and genetic modulation of GJIC on signal coordination remain unclear. This thesis investigates how pharmacological modulation via ouabain influences intracellular calcium signaling dynamics in MDCK-II cells stably expressing the genetically encoded calcium indicator GCaMP6m. Time-lapse fluorescence microscopy was used to capture calcium activity in untreated and treated cells. To analyze the data, a modular Python-based pipeline, extended from prior work, was employed. This tool, the Calcium Correlation Tool, integrates deep learning–based segmentation (via Cellpose), heuristic cell tracking, and correlation-based signal analysis within an interactive Napari environment. By combining automated and user-refinable steps, the pipeline enables reproducible quantification and visualization of intercellular calcium coordination. The tool's full source code was released as open source, supporting transparency and future reuse. By integrating live-cell imaging with computational signal analysis, this thesis contributes to a deeper understanding of collective calcium behavior in epithelial tissues and establishes a reproducible workflow for future studies on GJIC-dependent signaling mechanisms. ; Intercellulär kalciumsignalering spelar en avgörande roll i att samordna epitelcellers beteende vid vävnadsreparation, utveckling och sjukdomsprogression. Gap junction-kommunikation (GJIC), som främst förmedlas av connexinproteiner såsom connexin 43 (Cx43), möjliggör direkt molekylärt utbyte mellan intilliggande ...
Druh dokumentu: bachelor thesis
Popis souboru: application/pdf
Jazyk: English
Relation: TRITA-SCI-GRU; 2025:131
Dostupnost: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-365072
Rights: info:eu-repo/semantics/openAccess
Přístupové číslo: edsbas.BE086FC0
Databáze: BASE
Popis
Abstrakt:Intercellular calcium signaling plays a crucial role in coordinating epithelial cell behavior during tissue repair, development, and disease progression. Gap junctional intercellular communication (GJIC), primarily mediated by connexin proteins such as connexin 43 (Cx43), enables direct molecular exchange between adjacent cells. While previous studies have explored calcium signaling in MDCK-II epithelial cells and developed tools for visualizing intracellular calcium fluctuations, the specific effects of pharmacological and genetic modulation of GJIC on signal coordination remain unclear. This thesis investigates how pharmacological modulation via ouabain influences intracellular calcium signaling dynamics in MDCK-II cells stably expressing the genetically encoded calcium indicator GCaMP6m. Time-lapse fluorescence microscopy was used to capture calcium activity in untreated and treated cells. To analyze the data, a modular Python-based pipeline, extended from prior work, was employed. This tool, the Calcium Correlation Tool, integrates deep learning–based segmentation (via Cellpose), heuristic cell tracking, and correlation-based signal analysis within an interactive Napari environment. By combining automated and user-refinable steps, the pipeline enables reproducible quantification and visualization of intercellular calcium coordination. The tool's full source code was released as open source, supporting transparency and future reuse. By integrating live-cell imaging with computational signal analysis, this thesis contributes to a deeper understanding of collective calcium behavior in epithelial tissues and establishes a reproducible workflow for future studies on GJIC-dependent signaling mechanisms. ; Intercellulär kalciumsignalering spelar en avgörande roll i att samordna epitelcellers beteende vid vävnadsreparation, utveckling och sjukdomsprogression. Gap junction-kommunikation (GJIC), som främst förmedlas av connexinproteiner såsom connexin 43 (Cx43), möjliggör direkt molekylärt utbyte mellan intilliggande ...