Figure 2. Dendrite and spine calcium activity. ; (A) Mice were trained to lick either a right or left target based on pole location. The pole was within reach of the whiskers during the sample epoch. Mice were trained to withhold licks until after a delay and auditory response cue. (B) Top, example somatic (black), dendritic (magenta), and spine (green) calcium signals from a layer 2/3 example session (as shown Figure 1B–E). Bottom, maximum intensity projections (au) and branch insets at selected times (dashed vertical lines in upper traces). Note independent spine activity at time i. (C) Same as (B) but for the layer five example session (as in Figure 1F–I). The apical trunk (black) was targeted as a reference for global activity. Note branch-specific sustained activity at time ii.

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