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Single prokaryotic cell abundances of the Northeast Greenland (NEG) shelf sediments from POLARSTERN cruise PS109

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Bibliographic Details
Title: Single prokaryotic cell abundances of the Northeast Greenland (NEG) shelf sediments from POLARSTERN cruise PS109
Authors: Bodur, Yasemin V, Felden, Janine, Braeckman, Ulrike
Publisher Information: PANGAEA
Publication Year: 2024
Collection: PANGAEA - Data Publisher for Earth & Environmental Science (AWI Bremerhaven / MARUM Bremen)
Subject Terms: Acridine Orange Direct Counting (AODC), AODC, ARK-XXXI/4, B_LANDER, Bottom lander, Counts, Date/Time of event, Depth, bottom/max, sediment/rock, top/min, Elevation of event, Estimated from counts according to Hobbie et al. (1977), Event label, Filter, FRAM, FRontiers in Arctic marine Monitoring, Latitude of event, Longitude of event, Method/Device of event, Microbial abundance, cells, Multicorer with television, Number of cells, Polarstern, PS109, PS109_105-2, PS109_115-2, PS109_122-1, PS109_125-3
Subject Geographic: MEDIAN LATITUDE: 78.720554 * MEDIAN LONGITUDE: -14.878132 * SOUTH-BOUND LATITUDE: 76.486900 * WEST-BOUND LONGITUDE: -19.321920 * NORTH-BOUND LATITUDE: 80.317630 * EAST-BOUND LONGITUDE: -7.132010 * DATE/TIME START: 2017-09-17T15:16:00 * DATE/TIME END: 2017-10-07T02:06:00 * MINIMUM DEPTH, sediment/rock: 0.005 m * MAXIMUM DEPTH, sediment/rock: 0.045 m
Description: Samples for single cell abundances as an estimate for the abundance of benthic bacteria on the Northeast Greenland shelf have been collected with R/V Polarstern during PS109 between September and October 2017 using a camera-equipped multiple corer (TV-MUC; core area 0.007 m²). Upon arrival on deck, the upper 5 cm of three MUC cores were sliced into 1 cm layers with a 10 ml cut-off syringe. 2 ml of each slice was transferred into a scintillation vial and fixed with 2 % filtered formaldehyde-seawater solution. In the laboratory, the samples were diluted, filtered through polycarbonate filters (0.2 µm, Whatman Nucleopore Track-Etch Membrane) and stained with a 0.001 % acridine orange solution after Hobbie et al., 1977 (doi:10.1128/aem.33.5.1225-1228.1977). Cells were counted on at least 30 grids (125 µm * 125 µm area) for 2 replicate filters per sample each with a Zeiss Axiophot microscope (Germany) and a 100x oil immersion objective lens (Zeiss Plan-Apochromat, Germany).
Document Type: dataset
File Description: text/tab-separated-values, 1928 data points
Language: English
Relation: Bodur, Yasemin V; Renaud, Paul E; Lins, Lidia; Da Costa Monteiro, Luana; Ambrose, William G Jr; Felden, Janine; Krumpen, Thomas; Wenzhöfer, Frank; Włodarska-Kowalczuk, Maria; Braeckman, Ulrike (2024): Weakened pelagic-benthic coupling on an Arctic outflow shelf (Northeast Greenland) suggested by benthic ecosystem changes. Elementa - Science of the Anthropocene, 12(1), 00005, https://doi.org/10.1525/elementa.2023.00005; https://doi.pangaea.de/10.1594/PANGAEA.959552; https://doi.org/10.1594/PANGAEA.959552
DOI: 10.1594/PANGAEA.959552
Availability: https://doi.pangaea.de/10.1594/PANGAEA.959552
https://doi.org/10.1594/PANGAEA.959552
Rights: CC-BY-4.0: Creative Commons Attribution 4.0 International ; Access constraints: unrestricted ; info:eu-repo/semantics/openAccess
Accession Number: edsbas.A809D74A
Database: BASE
Description
Abstract:Samples for single cell abundances as an estimate for the abundance of benthic bacteria on the Northeast Greenland shelf have been collected with R/V Polarstern during PS109 between September and October 2017 using a camera-equipped multiple corer (TV-MUC; core area 0.007 m²). Upon arrival on deck, the upper 5 cm of three MUC cores were sliced into 1 cm layers with a 10 ml cut-off syringe. 2 ml of each slice was transferred into a scintillation vial and fixed with 2 % filtered formaldehyde-seawater solution. In the laboratory, the samples were diluted, filtered through polycarbonate filters (0.2 µm, Whatman Nucleopore Track-Etch Membrane) and stained with a 0.001 % acridine orange solution after Hobbie et al., 1977 (doi:10.1128/aem.33.5.1225-1228.1977). Cells were counted on at least 30 grids (125 µm * 125 µm area) for 2 replicate filters per sample each with a Zeiss Axiophot microscope (Germany) and a 100x oil immersion objective lens (Zeiss Plan-Apochromat, Germany).
DOI:10.1594/PANGAEA.959552