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Membrane on a Chip. A functional tethered lipid bilayer membrane on silicon oxide surface

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Title: Membrane on a Chip. A functional tethered lipid bilayer membrane on silicon oxide surface
Authors: Atanasov, V., Knorr, N., Duran, R. S., Ingebrandt, S., Offenhäusser, A., Knoll, W., Köper, I.
Source: Biophysical journal 89, 1780 - 1788 (2005). doi:10.1529/biophysj.105.061374
Publisher Information: Rockefeller Univ. Press
Publication Year: 2005
Collection: Forschungszentrum Jülich: JuSER (Juelich Shared Electronic Resources)
Subject Terms: info:eu-repo/classification/ddc/570, Biophysics: methods, Electric Impedance, Electrochemistry, Gold: chemistry, Gramicidin: pharmacology, Ion Channels: chemistry, Ionophores: pharmacology, Ions, Lipid Bilayers: chemistry, Lipid Bilayers: metabolism, Lipids: chemistry, Microscopy, Atomic Force, Models, Chemical, Phosphatidylcholines: chemistry, Phytol: chemistry, Protein Array Analysis: methods, Silanes: chemistry, Silanes: metabolism, Silicon: chemistry, Silicon Dioxide: chemistry, Spectrophotometry, Substrate Specificity, Temperature, Time Factors, Valinomycin: chemistry, Valinomycin: pharmacology, 2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-chloro-dimethylpropyl-silane) ether lipid
Subject Geographic: DE
Description: Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as functionality of a bilayer membrane. Here, we describe the synthesis of lipids for a more modular design and the adaptation of the linker part to silane chemistry. We were able to form a functional tethered bilayer lipid membrane with good electrical sealing properties covering a silicon oxide surface. We demonstrate the functional incorporation of the ion carrier valinomycin and of the ion channel gramicidin.
Document Type: article in journal/newspaper
Language: English
Relation: info:eu-repo/semantics/altIdentifier/pmid/pmid:16127170; info:eu-repo/semantics/altIdentifier/wos/WOS:000231502800034; info:eu-repo/semantics/altIdentifier/hdl/2128/2125; info:eu-repo/semantics/altIdentifier/issn/0006-3495
Availability: https://juser.fz-juelich.de/record/48738
https://juser.fz-juelich.de/search?p=id:%22PreJuSER-48738%22
Rights: info:eu-repo/semantics/openAccess
Accession Number: edsbas.9AFCDF3B
Database: BASE
Description
Abstract:Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as functionality of a bilayer membrane. Here, we describe the synthesis of lipids for a more modular design and the adaptation of the linker part to silane chemistry. We were able to form a functional tethered bilayer lipid membrane with good electrical sealing properties covering a silicon oxide surface. We demonstrate the functional incorporation of the ion carrier valinomycin and of the ion channel gramicidin.