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Development of an Aptamer-Based qPCR Method for the Selective and Rapid Picomolar-Level Detection of Perfluorooctanesulfonic Acid in Water

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Názov: Development of an Aptamer-Based qPCR Method for the Selective and Rapid Picomolar-Level Detection of Perfluorooctanesulfonic Acid in Water
Autori: Junyoung Park, Donghyun Kim, Dongyun Kim, Jihyeun Jung, Kyung-Duk Zoh, Changha Lee, Yongju Choi, Jong Kwon Choe
Rok vydania: 2025
Predmety: Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Biotechnology, Developmental Biology, Cancer, Inorganic Chemistry, Infectious Diseases, Environmental Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, various environmental media, prolonged human exposure, molecular dynamics simulations, important recognition epitopes, explored using truncation, dissociation constant (<, l ), indicating, based qpcr method, ), level detection capabilities, level detection, developed method, >< sub, situ <, k <, pfos ), widely recognized
Popis: Per- and polyfluoroalkyl substances (PFAS) are widely recognized as emerging contaminants because they are ubiquitous in various environmental media. Their potential for chronic toxicity after prolonged human exposure is a growing concern. Consequently, there is an urgent need to develop an appropriate technology to efficiently treat and rapidly and consistently monitor PFAS levels. This study reports the development of the first aptamers that can bind to perfluorooctanesulfonic acid (PFOS), with a dissociation constant ( K D ) of 6.76 μM, and exhibit a high specificity for PFOS even in the presence of other PFAS. The binding site and mechanism of the prepared aptamers are explored using truncation and molecular dynamics simulations, which show that the lengths of fluorocarbons and functional groups are important recognition epitopes. To demonstrate the application potential of the prepared aptamers, an aptamer-based quantitative polymerase chain reaction method is also developed, which exhibits picomolar-level detection capabilities and a limit of detection of 5.8 pM (2.9 ng/L), indicating its high sensitivity. Our findings demonstrate the potential of the developed method in the rapid in situ monitoring of PFOS at contamination sites, which will facilitate its early detection before rigorous analysis.
Druh dokumentu: article in journal/newspaper
Jazyk: unknown
DOI: 10.1021/acs.est.5c04730.s001
Dostupnosť: https://doi.org/10.1021/acs.est.5c04730.s001
https://figshare.com/articles/journal_contribution/Development_of_an_Aptamer-Based_qPCR_Method_for_the_Selective_and_Rapid_Picomolar-Level_Detection_of_Perfluorooctanesulfonic_Acid_in_Water/29852429
Rights: CC BY-NC 4.0
Prístupové číslo: edsbas.941DA1F0
Databáza: BASE
Popis
Abstrakt:Per- and polyfluoroalkyl substances (PFAS) are widely recognized as emerging contaminants because they are ubiquitous in various environmental media. Their potential for chronic toxicity after prolonged human exposure is a growing concern. Consequently, there is an urgent need to develop an appropriate technology to efficiently treat and rapidly and consistently monitor PFAS levels. This study reports the development of the first aptamers that can bind to perfluorooctanesulfonic acid (PFOS), with a dissociation constant ( K D ) of 6.76 μM, and exhibit a high specificity for PFOS even in the presence of other PFAS. The binding site and mechanism of the prepared aptamers are explored using truncation and molecular dynamics simulations, which show that the lengths of fluorocarbons and functional groups are important recognition epitopes. To demonstrate the application potential of the prepared aptamers, an aptamer-based quantitative polymerase chain reaction method is also developed, which exhibits picomolar-level detection capabilities and a limit of detection of 5.8 pM (2.9 ng/L), indicating its high sensitivity. Our findings demonstrate the potential of the developed method in the rapid in situ monitoring of PFOS at contamination sites, which will facilitate its early detection before rigorous analysis.
DOI:10.1021/acs.est.5c04730.s001