Generation and characterization of function-blocking anti-ectodysplasin A (EDA) monoclonal antibodies that induce ectodermal dysplasia.
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| Název: | Generation and characterization of function-blocking anti-ectodysplasin A (EDA) monoclonal antibodies that induce ectodermal dysplasia. |
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| Autoři: | Kowalczyk-Quintas, C., Willen, L., Dang, A.T., Sarrasin, H., Tardivel, A., Hermes, K., Schneider, H., Gaide, O., Donzé, O., Kirby, N., Headon, D.J., Schneider, P. |
| Rok vydání: | 2025 |
| Sbírka: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| Témata: | Animals, Antibodies, Monoclonal, Murine-Derived/genetics, Murine-Derived/immunology, Murine-Derived/toxicity, Neutralizing/genetics, Neutralizing/immunology, Neutralizing/toxicity, Autoantibodies/genetics, Autoantibodies/immunology, Autoantibodies/toxicity, Base Sequence, Cattle, Cell Line, Dogs, Ectodermal Dysplasia/chemically induced, Ectodermal Dysplasia/genetics, Ectodermal Dysplasia/immunology, Ectodermal Dysplasia/metabolism, Ectodermal Dysplasia/pathology, Ectodysplasins/antagonists & inhibitors, Ectodysplasins/genetics, Ectodysplasins/immunology, Ectodysplasins/metabolism, Female, Humans, Male, Mice, Mutant Strains |
| Popis: | Development of ectodermal appendages, such as hair, teeth, sweat glands, sebaceous glands, and mammary glands, requires the action of the TNF family ligand ectodysplasin A (EDA). Mutations of the X-linked EDA gene cause reduction or absence of many ectodermal appendages and have been identified as a cause of ectodermal dysplasia in humans, mice, dogs, and cattle. We have generated blocking antibodies, raised in Eda-deficient mice, against the conserved, receptor-binding domain of EDA. These antibodies recognize epitopes overlapping the receptor-binding site and prevent EDA from binding and activating EDAR at close to stoichiometric ratios in in vitro binding and activity assays. The antibodies block EDA1 and EDA2 of both mammalian and avian origin and, in vivo, suppress the ability of recombinant Fc-EDA1 to rescue ectodermal dysplasia in Eda-deficient Tabby mice. Moreover, administration of EDA blocking antibodies to pregnant wild type mice induced in developing wild type fetuses a marked and permanent ectodermal dysplasia. These function-blocking anti-EDA antibodies with wide cross-species reactivity will enable study of the developmental and postdevelopmental roles of EDA in a variety of organisms and open the route to therapeutic intervention in conditions in which EDA may be implicated. |
| Druh dokumentu: | article in journal/newspaper |
| Popis souboru: | application/pdf |
| Jazyk: | English |
| ISSN: | 1083-351X |
| Relation: | Journal of Biological Chemistry; https://iris.unil.ch/handle/iris/56163; serval:BIB_128AA3C5C25E; 000331403800038 |
| DOI: | 10.1074/jbc.M113.535740 |
| Dostupnost: | https://iris.unil.ch/handle/iris/56163 https://doi.org/10.1074/jbc.M113.535740 |
| Přístupové číslo: | edsbas.81144983 |
| Databáze: | BASE |
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Nájsť tento článok vo Web of Science | Abstrakt: | Development of ectodermal appendages, such as hair, teeth, sweat glands, sebaceous glands, and mammary glands, requires the action of the TNF family ligand ectodysplasin A (EDA). Mutations of the X-linked EDA gene cause reduction or absence of many ectodermal appendages and have been identified as a cause of ectodermal dysplasia in humans, mice, dogs, and cattle. We have generated blocking antibodies, raised in Eda-deficient mice, against the conserved, receptor-binding domain of EDA. These antibodies recognize epitopes overlapping the receptor-binding site and prevent EDA from binding and activating EDAR at close to stoichiometric ratios in in vitro binding and activity assays. The antibodies block EDA1 and EDA2 of both mammalian and avian origin and, in vivo, suppress the ability of recombinant Fc-EDA1 to rescue ectodermal dysplasia in Eda-deficient Tabby mice. Moreover, administration of EDA blocking antibodies to pregnant wild type mice induced in developing wild type fetuses a marked and permanent ectodermal dysplasia. These function-blocking anti-EDA antibodies with wide cross-species reactivity will enable study of the developmental and postdevelopmental roles of EDA in a variety of organisms and open the route to therapeutic intervention in conditions in which EDA may be implicated. |
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| ISSN: | 1083351X |
| DOI: | 10.1074/jbc.M113.535740 |